Objective: To establish an HPLC method for the simultaneous determination of twelve chemical compositions including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isovitexin, forsythiaside A, luteoloside, verbascoside, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, and linarin in Xiaoer Ganmao Granules. Methods: The ACE Excel 5 C18-AR column (4.6 mm×250 mm, 5 μm) was used, the mobile phase was acetonitrile(A)-0.1% glacial acetic acid(B) with gradient elution at the flow rate of 1.0 mL·min-1, the detection wavelength was 330 nm, and the column temperature was at 30 ℃. Results: The linear relationship of the twelve chemical components was good,and the r values were all above 0.999 1.Results of precision (RSD≤0.8), repeatability (RSD≤1.1) and stability (RSD≤1) were good. The average recoveries were 96.0%-100.6% with RSDs of 0.56%-1.8%. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isovitexin, forsythiaside A, luteoloside, verbascoside, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, and linarin in eleven batches of samples were 0.044-0.111, 0.059-0.097, 0.082-0.143, 0.006-0.039, 0.204-0.896, 0.093-0.617, 0.002-0.233, 0.012-0.049, 0.03-0.117, 0.042-0.162, 0.044-0.111 and 0.059-0.097 mg·g-1, respectively. Conclusion: This method is specific, accurate and reproducible, which can be used for determining the contents of ten constituents in Xiaoer Ganmao Granules.
TANG Jun, YUAN Ming-ming, ZHOU Guo-ping, YANG Qun
. Simultaneous determination of twelve chemical compositions in Xiaoer Ganmao Granules by HPLC[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(12)
: 2179
-2185
.
DOI: 10.16155/j.0254-1793.2022.12.15
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