Objective: To establish an HPLC method for the simultaneous determination of eight components including salvianic acid A sodium, geniposidic acid, phellodendrine, salvianolic acid B, palmatine, berberine, atractylodin, tanshinone ⅡA in Shuangshi Tonglin capsules. Methods: The determination was performed on a Capcell Pak MG Ⅱ C18 column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile-water(containing 0.1% phosphoric acid)-water(containing 0.1% phosphoric acid and 0.2% triethylamine, pH 6.0) in a gradient mode at a flow rate of 1.0 mL·min-1. The column temperature was set at 30 ℃,the injection volume was 10 μL, and the detection wavelength was 280 nm (0-13.4 min)for salvianic acid A sodium, 238 nm (13.5-17.0 min) for geniposidic acid, 280 nm (17.1-51.0 min)for phellodendrine, salvianolic acid B, 345 nm(51.1.1-70.0 min)for palmatine, berberine, 336 nm(70.1-78.0 min)for atractylodin and 268 nm(78.1-90.0 min)for tanshinone ⅡA. Results: Salvianic acid A sodium, geniposidic acid, phellodendrine, salvianolic acid B, palmatine, berberine, atractylodin, tanshinoneⅡA had good linearities in their respective linear ranges with all linear correlation coefficients ≥ 0.999 5 and RSDs of the precision, repeatability and stability less than 2.0%. The average recoveries were 101.3%, 98.2%, 97.3%, 98.1%, 96.6%, 97.9%, 98.7%, 99.6%, with RSDs lower than 2.0%. The contents of the above eight components in three batches of samples was 4.47-5.51 mg·g-1, 0.93-1.44 mg·g-1, 1.00-1.07 mg·g-1, 5.72-8.67 mg·g-1, 0.64-0.69 mg·g-1, 1.62-1.90 mg·g-1, 0.055-0.088 mg·g-1, 0.29-0.44 mg·g-1, respectively. Conclusion: The proposed method is rapid, sensitive and specific, which can be used for determination the contents of eitht components in Shuangshi Tonglin capsules.
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