活性分析

青翘、连翘叶多糖的分离纯化、结构解析和抗炎活性比较*

  • 赵小艳 ,
  • 高秀华 ,
  • 申晓芸 ,
  • 李科 ,
  • 李震宇
展开
  • 1.山西药科职业学院,太原 030031;
    2.山西大学中医药现代研究中心,太原 030006;
    3. 山西大学化学生物学与分子工程教育部重点实验室,太原 030006;
    4.地产中药功效物质研究与利用山西省重点实验室,太原 030006
第一作者 Tel:15615237219;E-mail:2284667690@qq.com
** Tel:(0351)7011202;E-mail:like@sxu.edu.cn

收稿日期: 2024-12-07

  网络出版日期: 2025-08-25

基金资助

*2024年度山西省中医药管理局科研课题(2024ZYYB055)连翘多糖分离纯化、结构解析及体外抗炎活性成分筛选

Separation, purification, structural analysis, and comparison of the anti-inflammatory activity of polysaccharides in Green Fructus Forsythiae and Folium Forsythiae*

  • ZHAO Xiao-yan ,
  • GAO Xiu-hua ,
  • SHEN Xiao-yun ,
  • LI Ke ,
  • LI Zhen-yu
Expand
  • 1. Shanxi Pharmaceutial Vocational College, Taiyuan 030031, China;
    2. Modern Research Center of Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
    3. Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Shanxi University, Taiyuan 030006, China;
    4. Shanxi Provincial Key Laboratory of Research and Utilization of Functional Substances in Traditional Chinese Medicine, Taiyuan 030006, China

Received date: 2024-12-07

  Online published: 2025-08-25

摘要

目的: 从青翘以及连翘叶中提取分离得到青翘多糖和连翘叶多糖进行结构表征和抗炎活性比较。方法: 通过浸提和纯化工艺获得粗多糖,采用凝胶排阻色谱和激光光散射检测法测定其分子量,使用MTT法评估了多糖的细胞毒性,通过ELISA法测定NO、TNF-α和IL-10的水平。结果: 聚合物分散性指数(PDI)显示其分子量分布较为均匀。单糖组成分析表明,2种多糖均含有多种单糖,其中青翘多糖含有更多的半乳糖醛酸,而连翘叶多糖葡萄糖含量较高。甲基化分析显示青翘多糖主要通过4-Gal(p)-UA连接,而连翘叶多糖则主要通过4-Glc(p)连接。青翘和连翘叶多糖均能显著抑制LPS诱导的NO和TNF-α的产生,同时促进IL-10的生成,且连翘叶多糖在这些效应上优于青翘多糖。结论: 青翘与连翘叶多糖具有显著的抗炎活性,本研究为连翘资源开发奠定基础。

本文引用格式

赵小艳 , 高秀华 , 申晓芸 , 李科 , 李震宇 . 青翘、连翘叶多糖的分离纯化、结构解析和抗炎活性比较*[J]. 药物分析杂志, 2025 , 45(3) : 440 -451 . DOI: 10.16155/j.0254-1793.2024-1320

Abstract

Objective: To extract and isolate polysaccharides from Green Fructus Forsythiae and Folium Forsythiae for structural characterization and comparison of their anti-inflammatory activities. Methods: Crude polysaccharides were obtained through extraction and purification processes. Gel permeation chromatography and laser light scattering were used to determine their molecular weight. The MTT assay was employed to evaluate the cytotoxicity of the polysaccharides, while ELISA was used to measure levels of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-10 (IL-10). Results: The polydispersity index (PDI) indicated a relatively uniform molecular weight distribution. Monosaccharide composition analysis showed that both polysaccharides contained various monosaccharides, with Green Fructus Forsythiae polysaccharide having a higher content of galacturonic acid, while Folium Forsythiae polysaccharide had a higher glucose content. Methylation analysis revealed that polysaccharides in Green Fructus Forsythiae were primarily linked by 4-Gal (p)-UA, while those of Folium Forsythiae polysaccharide were mainly linked by 4-Glc (p). Both polysaccharides significantly inhibited the production of NO and TNF-α induced by LPS while promoting the generation of IL-10, with polysaccharide in Folium Forsythiae showing superior effects compared to Green Fructus Forsythiae polysaccharide. Conclusion: Polysaccharides from Green Fructus Forsythiae and Folium Forsythiae exhibit significant anti-inflammatory activities. This research lays the foundation for the development of Folium Forsythiae resources.

参考文献

[1] WANG ZY,XIA Q,LIU X,et al.Phytochemistry,pharmacology,quality control and future research of Forsythia suspensa(Thunb.)Vahl:a review[J].J Ethnopharmacol,2018,210:318
[2] 宋小俊. 连翘不同部位化学成分研究进展[J].西北药学杂志,2014,29(2):220
SONG XJ.Research advances of chemical composition of different parts of Forsythia suspensa[J].Northwest Pharm J,2014,29(2):220
[3] WANG LX,WANG HL,HUANG J,et al.Review of lignans from 2019 to 2021:newly reported compounds,diverse activities,structure-activity relationships and clinical applications[J].Phytochemistry,2022,202:113326.
[4] XIAO ZY,GUO YZ,LI JX,et al.Harnessing traditional Chinese medicine polysaccharides for combatting COVID-19[J].Carbohydr Polym,2024,346:122605.
[5] 山西省卫生和计划生育委员会,山西省食品药品监督管理局.DBS 14/001-2017食品安全地方标准连翘叶[S].2017
Shanxi Provincial Health and Family Planning Commission,Shanxi Provincial Food and Drug Administration.DBS 14/001-2017 Local Standards for Food Safety:Forsythia Leaves[S].2017
[6] 王燕,王儒彬,孙磊,等.不同采摘期连翘叶中总黄酮、总酚酸含量与DPPH自由基清除能力的相关性[J].中国实验方剂学杂志,2011,17(16):109
WANG Y,WANG RB,SUN L,et al.relationship between total flavonoids,total phenolics and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities during growth of Forsythia suspensa leaves[J].Chin J Exp Tradit Med Form,2011,17(16):109
[7] GUI LL,WANG SK,WANG J,et al.Effects of forsythin extract in Forsythia leaves on intestinal microbiota and short-chain fatty acids in rats fed a high-fat diet[J].Food Sci Hum Wellness,2024,13(2):659
[8] DU BX,YEPEI FU YP,WANG X,et al.Isolation,purification,structural analysis and biological activities of water-soluble polysaccharide from Glehniae Radix[J].Int J Biol Macromol,2019,128:724
[9] ZHANG H,LICC,LAI P F H,et al.Fractionation,chemical characterization and immunostimulatory activity of β-glucan and galactoglucan from Russula vinosa Lindblad[J].Carbohydr Polym,2021,256:117559
[10] HU T,HUANG Q,WONG K,et al.Structure,molecular conformation,and immunomodulatory activity of four polysaccharide fractions fro Lignosus rhinocerotis sclerotia[J].Int J Biol Macromol,2017,94:423
[11] ZHENG ZM,HUANG QL,LING CQ.Water-soluble yeast β-glucan fractions with different molecular weights:extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity[J].Int J Biol Macromol,2019,123:269
[12] WANG L,ZHANGB,XIAO J,et al.Physicochemical,functional,and biological properties of water-soluble polysaccharides from Rosa roxburghii Tratt fruit[J].Food Chem,2018,249:127
[13] ZHU M,HUANG R,WEN P,et al.Structural characterization and immunological activity of pectin polysaccharide from kiwano(Cucumis metuliferus)peels[J].Carbohydr Polym,2021,254:117371
[14] LIU H,XU J,XU X,et al.Structure/function relationships of bean polysaccharides:a review[J].Crit Rev Food Sci Nutr,2023,63(3):330
[15] LIU T,REN Q,WANG S,et al.Chemical modification of polysaccharides:a review of synthetic approaches,biological activity and the structure-activity relationship[J].Molecules,2023,28(16):6073
[16] LIN JF,XIANG SW,LV H,et al.Antimicrobial high molecular weight pectin polysaccharides production from diverse citrus peels using a novel PL10 family pectate lyase[J].Int J Biol Macromol,2023,234:123457
[17] LU J,HE R,SUN P,et al.Molecular mechanisms of bioactive polysaccharides from Ganoderma lucidum(Lingzhi),a review[J].Int J Biol Macromol,2020,150:765
[18] LIU G,ZHANG J,KAN Q,et al.Extraction,structural characterization,and immunomodulatory activity of a high molecular weight polysaccharide from Ganoderma lucidum[J].Front Nutr,2022,9:846080.DOI:10.3389/fnut.2022.846080
[19] WANG ZC,ZHENG Y,LAI ZR,et al.Effect of monosaccharide composition and proportion on the bioactivity of polysaccharides:A review[J].Int J Biol Macromol,2024,254.DOI:10.1016/j.ijbiomac.2023.127955
[20] LI WZ,YANG BX,JOE G H,et al.Glycation with uronic acid-type reducing sugar enhances the anti-inflammatory activity of fish myofibrillar protein via the Maillard reaction[J].Food Che,2023,407:135162
[21] DU B,LIN CY,BIAN ZX,et al.An insight into anti-inflammatory effects of fungal beta-glucans[J].Trends Food Sci Technol,2015,41(1):49
[22] CHEN N,JIANG TY,XU JX,et al.The relationship between polysaccharide structure and its antioxidant activity needs to be systematically elucidated[J].Int J Biol Macromo,2024,270:132391
[23] SUN X,STEFANETTI G,BERTI F,et al.Polysaccharide structure dictates mechanism of adaptive immune response to glycoconjugate vaccines[J].Proc Natl Acad Sci U S A,2019,116(1):193
[24] CHEN GM,JIANG N,ZHENG JP,et al.Structural characterization and anti-inflammatory activity of polysaccharides from Astragalus membranaceus[J].Int J Biol Macromo,2023,241:124386
[25] ZENG F,LI Y,ZHANG X,et al.Immune regulation and inflammation inhibition of Arctium lappa L.polysaccharides by TLR4/NF-κB signaling pathway in cells[J].Int J Biol Macromol,2024254(Pt2):127955
[26] ZHANG Y,YAN M,XIA Y,et al.Glutaredoxin-1 modulates the NF-κB signaling pathway to activate inducible nitric oxide synthase in experimental necrotizing enterocolitis[J].Mol Ther Methods Clin Dev,2024,32(1):101214
文章导航

/