HPLC Fingerprint and chemical pattern recognition of Andrographis Herba with different growth period

doi:10.16155/j.0254-1793.2021.03.05

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Chinese Journal of Pharmaceutical Analysis ›› 2021, Vol. 41 ›› Issue (3) : 410-420. DOI: 10.16155/j.0254-1793.2021.03.05

Ingredient Analysis

HPLC Fingerprint and chemical pattern recognition of Andrographis Herba with different growth period

WANG Zhen

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Abstract

Objective: To establish an HPLC fingerprint of Andrographis Herba in different growth period, and to evaluate the quality through chemical pattern recognition. Methods: The analysis was performed on Agilent Eclipse XDB-C₁₈（250 mm×4. 6 mm, 5 μm））column with mobile phase consisted of acetonitrile-0. 2% phosphoric acid solution（gradient elution）at a flow rate of 1. 0 mL·min^-1. The detection wavelengths were set at 225 and 250 nm. The fingerprint common pattern and similarity calculation were established using traditional Chinese medicine chromatographic fingerprint similarity evaluation system. The cluster analysis(CA）, principal component analysis （PCA）, and orthogonal partial least square discriminate analysis(OPLS-DA） models were established using SPSS22. 0 and SIMCA-P14. 1 software to perform statistical analysis. Results: HPLC fingerprint of Andrographis Herba was established successfully and 17 common peaks were identified in the study, 4 chemical compounds were identified using standard references. The similarity calculation results were 0. 902~0. 997. PCA showed that the optimum harvesting stage of Andrographis Herba in different growth period was early flowering stage and PLSDA screened out 5 differential markers. Conclusion: The HPLC fingerprint can reflect the differences in different growth period, combining PCA and PLS-DA can provide reference for the harvesting and quality evaluation of Andrographis Herba.

Key words

Andrographis Herba / HPLC fingerprint / similarity / chemical pattern recognition / principal component analysis / orthogonal partial least square discriminate analysis

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WANG Zhen. HPLC Fingerprint and chemical pattern recognition of Andrographis Herba with different growth period[J]. Chinese Journal of Pharmaceutical Analysis, 2021, 41(3): 410-420 https://doi.org/10.16155/j.0254-1793.2021.03.05

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