Establishment of identification method for Fritillariae Cirrhosae Bulbus and its adulterant Fritillariae Ussuriensis Bulbus based on chemometrics

doi:10.16155/j.0254-1793.2023-0616

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Chinese Journal of Pharmaceutical Analysis ›› 2024, Vol. 44 ›› Issue (7) : 1222-1232. DOI: 10.16155/j.0254-1793.2023-0616

Safety Monitoring

Establishment of identification method for Fritillariae Cirrhosae Bulbus and its adulterant Fritillariae Ussuriensis Bulbus based on chemometrics

SHI Yan, LIU Wei, WEI Feng^*, MA Shuang-cheng^*

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Abstract

Objective: To establish a method for identifying Fritillariae Cirrhosae Bulbus and its adulterant Fritillariae Ussuriensis Bulbus. Methods: Chemometrics techniques were used for the analysis of the sample data from determination with Q TOF MS, and characteristic ion pairs were selected as m/z 578.3→164.14 and m/z 578.3→398.31 with triple quadrupole mass spectrometry applied, respectively. Then a specific approach was developed which used Waters Acquisition UPLC CSH (75 mm×2.1 mm, 1.7 μm) as column, and with acetonitrile and 0.1% formic acid solution as the mobile phase, and a flow rate of 0.4 mL·min^-1. Two ion pairs: m/z 578.3→164.14 and m/z 578.3→398.31 were detected by triple quadrupole mass spectrometer with MRM mode. Results: The validation results through 108 batches of samples indicated that the two characteristic ion pairs, m/z 578.3→164.14 and m/z 578.3→398.31, could effectively distinguish between Fritillariae Cirrhosae Bulbus and its adulterant Fritillariae Ussuriensis Bulbus. Conclusion: The method had good specificity and sensitivity, and could be used for the detection of adulterated Fritillariae Cirrhosae Bulbus with Fritillariae Ussuriensis Bulbus.

Key words

Fritillariae Cirrhosae Bulbus / Fritillariae Ussuriensis Bulbus / chemometrics / OPLS-DA / counterfeit identification / LC-MS method / data analysis / feature screening / characteristic ion

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SHI Yan, LIU Wei, WEI Feng, MA Shuang-cheng. Establishment of identification method for Fritillariae Cirrhosae Bulbus and its adulterant Fritillariae Ussuriensis Bulbus based on chemometrics[J]. Chinese Journal of Pharmaceutical Analysis, 2024, 44(7): 1222-1232 https://doi.org/10.16155/j.0254-1793.2023-0616

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