Simultaneous determination of 5 components in Physochlaina infundibularis Kuang by QAMS*

doi:10.16155/j.0254-1793.2023-0816

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Chinese Journal of Pharmaceutical Analysis ›› 2024, Vol. 44 ›› Issue (9) : 1604-1612. DOI: 10.16155/j.0254-1793.2023-0816

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Simultaneous determination of 5 components in Physochlaina infundibularis Kuang by QAMS^*

ZHANG Yu-ru, ZHANG Hong, MENG Xue, ZHANG Yu, WANG Di, CHEN Juan^**

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Abstract

Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method to simultaneously determine the contents of scopolamine, anisodamine, scopolin, hyoscyamine and scopoletin in Physochlaina infundibularis Kuang. Methods: Agilent 5 TC-C₁₈ (250 mm×4.6 mm, 5 μm) column was used with methanol-0.1% phosphoric acid aqueous solution as mobile phase for gradient elution at a flow rate of 1.0 mL·min^-1.The detection wavelengths were 210 nm and 344 nm, the column temperature was 30 °C, and the injection volume was 10 μL. The relative correction factors for scopolamine, scopolin, hyoscyamine and scopoletin and anisodamine were calculated by using anisodamine as the internal reference substance. The contents were determined and compared with the results of external standard method (ESM). Results: The five components had good linear relationship in their respective ranges(r=1.000). The relative correction factors of scopolamine, anisodamine, scopolin, hyoscyamine and scopoletin were 0.951 8, 0.562 6, 0.830 4 and 0.433 9, respectively, with good repeatability. The contents of scopolamine, anisodamine, scopolin, hyoscyamine and scopoletin in P. infundibularis from 10 habitats calculated by QAMS method were not significantly different from those determined by ESM. Conclusion: The method is accurate, stable and reproducible, and can be used for the quality control of P. infundibularis.

Key words

Physochlaina infundibularis Kuang / alkaloid / multi-components by single marker (QAMS) / content determination / relative correction factors (RCF)

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ZHANG Yu-ru, ZHANG Hong, MENG Xue, ZHANG Yu, WANG Di, CHEN Juan. Simultaneous determination of 5 components in Physochlaina infundibularis Kuang by QAMS^*[J]. Chinese Journal of Pharmaceutical Analysis, 2024, 44(9): 1604-1612 https://doi.org/10.16155/j.0254-1793.2023-0816

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