Study on UPLC fingerprint and quantitative analysis of multi-components by single marker of Qiye Shen'an dropping pills*

doi:10.16155/j.0254-1793.2024.03.03

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Chinese Journal of Pharmaceutical Analysis ›› 2024, Vol. 44 ›› Issue (3) : 386-394. DOI: 10.16155/j.0254-1793.2024.03.03

Ingredient Analysis

Study on UPLC fingerprint and quantitative analysis of multi-components by single marker of Qiye Shen'an dropping pills^*

WANG Jing-hui¹, YU Mi-mi¹, Chen Jing¹, WANG An-qi¹, FANG Shuang², CHEN You-gen¹, ZHANG Yun-sheng³, FU Xin-tong^1**, GUO Hong-zhu^1**

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Abstract

Objective: To establish the ultra high performance liquid chromatography (UPLC) fingerprint of Qiye Shen'an dropping pills, and to determine the contents of 8 index components by quantitative analysis of multi-components by single marker at the same time. Methods: Waters CORTECS^® T3 (150 mm×2.1 mm, 1.6 μm) chromatography column was used for gradient elution with acetonitrile-0.1% phosphoric acid as mobile phase, flow rate was 0.40 mL·min^-1, column temperature was 35 ℃, detection wavelength was 203 nm. Results: The UPLC fingerprint of Qiye Shen'an dropping pills was established, 8 common peaks were established, and the similarity of 15 batches of samples was greater than 0.99. Ginsenoside Rb₁, ginsenoside RC, panax notoginseng saponin FC, ginsenoside Rb₂, ginsenoside Rb₃, ginsenoside Rd, panax notoginseng saponin Fe and panax notoginseng saponin Fd were determined by quantitative analysis of multi-components by single marker method. The contents of 8 components in 15 batches were between 7.6-10.0 mg per pill. The average recoveries were 99.8%, 100.6%, 99.8%, 101.2%, 102.1%, 101.9%, 99.7% and 100.2% respectively, and the RSDs were 2.3%, 2.2%, 2.0%, 1.5%, 1.7%, 1.2%, 2.4% and 2.6% respectively. Conclusion: The UPLC fingerprint of Qiye Shen'an dropping pills and the content determination method of quantitative analysis of multi-components by single marker method established in this study are simple to operation, and have good repeatability, stability and reliability, and can provide a more comprehensive basis for the quality control of Qiye Shen'an dropping pills.

Key words

Qiye Shen'an dropping pills / total saponins from panaxnotpginseng stem and leaf / ginsenoside / panax notoginseng saponin / UPLC / fingerprint / quantitative analysis of multi-components by single marker / quality control

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WANG Jing-hui, YU Mi-mi, Chen Jing, WANG An-qi, FANG Shuang, CHEN You-gen, ZHANG Yun-sheng, FU Xin-tong, GUO Hong-zhu. Study on UPLC fingerprint and quantitative analysis of multi-components by single marker of Qiye Shen'an dropping pills^*[J]. Chinese Journal of Pharmaceutical Analysis, 2024, 44(3): 386-394 https://doi.org/10.16155/j.0254-1793.2024.03.03

References

[1] 杨晓艳. 小剂量多塞平片联合七叶神安滴丸治疗失眠伴焦虑疗效观察[J]. 中医临床研究, 2015, 7 (7): 12
YANG XY. Effective observation on treating insomnia with anxiety with doxepin tablets plus the Qiye Shenan pill[J]. Clin J Chin Med, 2015, 7 (7): 12
[2] 马妮, 赵爱, 曾鸿超, 等. 七叶神安滴丸长期毒性试验研究[J]. 现代中药研究与实践, 2011, 25(6): 46
MAN, ZHAO A, ZENG HC, et al. Study on long-term toxicity of Qiyeshenan Diwan[J]. Res Pract Chin Med, 2011, 25(6): 46
[3] 高维明, 周原, 任小宇. 三七茎叶皂苷对异丙肾上腺素诱导大鼠急性心肌缺血的保护作用[J]. 现代药物与临床, 2019, 34(6): 1615
GAO WM, ZHOU Y, REN XY. Protection of Panax notoginsegnoside of leaves on acute myocardial ischemia induced by isoprenaline in rats[J]. Drugs Clin, 2019, 34(6): 1615
[4] 王钢, 陈琼芳, 李钊飞, 等. 三七总皂苷及其主要成分对脑细胞保护作用的机制研究进展[J]. 中华中医药学刊, 2017, 35(7): 1743
WANG G, CHEN QF, LI ZF, et al. Study progress on mechanism of protective effect of PNS and its major components on brain cell[J]. Chin Arch Tradit Chin Med, 2017, 35(7): 1743
[5] 余佳丽, 唐晓章, 周菲, 等. 三七药材-三七二醇皂苷指纹图谱及其物质群量值传递分析[J]. 中国实验方剂学杂志, 2018, 24(7): 18
YU JL,TANG XZ, ZHOU F, et al. Analysis of fingerprint of Notoginseng Radix et Rhizoma-panaxadiol saponins and their quality transmitting of components group[J]. Chin J Exp Tradit Med Form, 2018, 24(7): 18
[6] 黎江华, 刘玉杰, 黄永亮, 等. 一测多评法测定熟三七中7种皂苷类成分的含量[J]. 时珍国医国药, 2017, 28 (10): 2363
LI JH, LIU YJ, HUANG YL, et al. Simultaneous determination of seven saponins in steamed Panax notoginseng by quantitative analysis of multi-components by single marker[J]. Lishzhen Med Mater Med Res, 2017, 28 (10): 2363
[7] 孟祥松, 蒋磊, 于现花, 等. 三七花及其易混淆品HPLC指纹图谱鉴别研究[J]. 辽宁中医药大学学报, 2018, 20(6): 8
MENG XS,JIANG L, YU XH, et al. Identification of notoginseng flower and its adulterants by HPLC fingerprint chromatogram[J]. J Liaoning Univ Tradit Chin Med, 2018, 20(6): 8
[8] 张迅杰, 赫军, 柳芳, 等. 人参和三七HPLC特征图谱研究[J]. 山东中医药大学学报, 2017, 41(4): 372
ZHANG XJ, HAO J, LIU F, et al. Study on HPLC characteristic fingerprint of Panax Ginseng Radix C. A. Mey. and Panax notoginseng(Burk.) F. H. Chen[J]. J Shandong Univ Tradit Chin Med, 2017, 41(4): 372
[9] 毕晓伟, 张璐璐, 张艳. 一测多评法同时测定三七伤药片中6种活性成分[J]. 中国药师, 2018, 21(8): 1490
BI XW, ZHANG LL, ZHANG Y. Determination of six active constituents in Sanqi Shangyao tablets by quantitative analysis of multi-components by single marker[J]. Chin Pharm, 2018, 21(8): 1490
[10] 刘阳, 段志敏, 熊宏苑, 等. 云南文山鲜三七花、茎、叶、根中人参皂甙成分测定[J]. 食品安全质量检测学报, 2017, 8(10): 3864
LIU Y, DUAN ZM, XIONG HY, et al. Determination of ginsenosides in flower, stem, leaf and root of fresh Panax notoginseng from Wenshan, Yunnan province[J]. J Food Safet Qual, 2017, 8(10): 3864
[11] 吴芸. 一测多评法测定血塞通注射液中5种成分含量[J]. 中医药导报, 2018, 24(11): 66
WU Y. Quantitative analysis of 5 components in Xuesaitong injection by QAMS[J]. Guid J Tradit Chin Med Pharmacol, 2018, 24(11): 66
[12] 黄朝蓉, 杨兆祥. 三七茎叶中皂苷成分及药理作用研究进展[J]. 中国民族民间医药, 2022, 31(23): 51
HYANG CR, YANG ZX. Advances in studies on constituents and pharmacological effects of saponins in stem lesves of Panax notoginseng[J]. Chin J Ethnomed Ethnopharm, 2022, 31(23): 51
[13] 李瑞明, 杨梦雅, 罗学军, 等. 三七叶中人参皂苷Rb₃及总皂苷质量差异研究[J]. 天津药学, 2021, 33(4): 1
LI RM, YANG MY, LUO XJ, et al. Study on the difference of ginsenoside Rb₃ and total saponins in the leaves of Panax notoginseng[J]. Tianjin Pharm, 2021, 33(4): 1
[14] 林灵超, 林雪志, 周小靖. 高效液相色谱法快速测定七叶神安片中的人参皂苷Rb₃[J]. 化学分析计量, 2019, 28(6): 66
LIN LC, LIN XZ, ZHOU XJ. Rapid determination of ginsenoside Rb₃ in Qiyeshen'an tablets by HPLC[J]. Chem Anal Meter, 2019, 28(6): 66
[15] 雷蓉, 赵振霞, 刘永利, 等. 超高效液相色谱法同时测定三七的叶和花中3个皂苷类成分含量[J]. 药物分析杂志, 2018, 38(7): 1146
LEI R, ZHAO ZX, LIU YL, et al. Simultaneous determination of three saponins in leaves and flower buds of Panax notoginseng by UPLC[J]. Chin J Pharm Anal, 2018, 38(7): 1146
[16] 沙孟晨, 周永峰, 张海珠, 等. 三七不同部位化学成分的差异性研究[J]. 中国现代中药, 2018, 20(7): 832
SHA MC, ZHOU YF, ZHANG HZ, et al. Study on differences of chemical components in different parts of Panax notoginseng [J]. Mod Chin Med, 2018, 20(7): 832
[17] 鞠康, 胡云飞, 徐倩, 等. 三七花质量控制方法研究进展[J]. 齐齐哈尔医学院学报, 2018, 39(12): 1425
JIU K, HU YF, XU Q, et al. The research progress of Panax notoginseng flower quality control methods[J]. J Qiqihar Univ Med, 2018, 39(12): 1425
[18] 赵振霞, 王敏, 段吉平, 等. 三七叶、三七花质量标准研究[J]. 中国现代中药, 2018, 20(8): 979
ZHAO ZX, WANG M, DUAN JP, et al. Quality specification study of leaves and flower buds of Panax notoginseng[J]. Mod Chin Med, 2018, 20(8): 979
[19] 赵振霞, 王敏, 王晓蕾, 等. 心可舒胶囊中非法添加三七茎叶的UPLC/MS/MS法检测[J]. 时珍国医国药, 2019, 30(3): 619
ZHAO ZX, WANG M, WANG XL, et al. Determination of Panax notoginseng leaves illegally added in Xinkeshu capsules by UPLC/MS/MS[J]. Lishenzhen Med Mater Med Res, 2019, 30(3): 619
[20] 陈玉勇, 秦枫, 朱善元, 等. 液相色谱-串联质谱检测田七花总皂苷中三七皂苷R₁的含量[J]. 江苏农业科学, 2014, 42(12): 319
CHEN YY, QIN F, ZHU SY, et al. Determination notoginsenoside R₁ in the total saponins of Tianqi flowers by UPLC-MS/MS[J]. Jiangsu Agr Sci, 2019, 42(12): 319