Review & Monography

Progress of next generation sequencing technology and its application and development trend in drug quality control*

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  • 1. Suzhou Institute for Drug Control,Suzhou 215104,China;
    2. Suzhou Municipal Hospital,Suzhou 215000,China;
    3. Ningxia Medical University,Yinchuan 750004,China

Received date: 2020-01-18

  Online published: 2024-05-31

Abstract

Innovation and wide application of gene sequencing technology have significantly promoted the development of medicine and life science. Since Sanger sequencing and chemical degradation were invented as the first generation sequencing technology,gene sequencing technology has been developed into the fourth generation in the past 40 years. The first generation sequencing was based on Sanger sequencing and Maxam-Gilbert method. These methods showed long sequencing length and high accuracy,but with high cost,low throughput and long sequencing time. The main platforms of the second generation sequencing included 454/GS-FLX,SOLID,Solexa, Hiseq/Miseq,and Heliscope platforms. The third generation sequencing platform was based on single molecule level side synthesis and side sequencing without PCR amplification,mainly including Heliscope platform and SMRT platform. ONT nanopore single molecule sequencing technology was based on electrical signal rather than optical signal. This method had the advantages of high throughput,low cost,short time and relatively simple data analysis,which was usually classified as the fourth generation sequencing platform. Sequencing technology is widely used in environment control of drug and quality analysis of traditional Chinese medicine. With the development of sequencing technology and bioinformatics,the application of sequencing technology in medicine and life science will play a more significant role.

Cite this article

ZHANG Guo-lin, JING Rong-xian, LIU Kun-mei, ZHOU Qin, GUO Le, XING Yi-wen, XUE Man . Progress of next generation sequencing technology and its application and development trend in drug quality control*[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(1) : 1 -12 . DOI: 10.16155/j.0254-1793.2021.01.01

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