Objective: To prepare the corresponding physical products of Ganjiang Lingshu decoction,in order to establish a method for HPLC content determination and fingerprinting of the corresponding physical products of Ganjiang Lingzhu decoction. Methods: According to the ancient books,Ganjianglingzhu decoction was prepared, the contents of active components and fingerprint of 15 corresponding materials were established by HPLC,the mobile phase was eluted with acetonitrile-0.05% phosphate water gradient,and the detection wavelength was 237 nm. Results: The HPLC content determination and fingerprint analysis method of Ganjiang Lingshu decoction was established. The peak resolutions in the specificity test of glycyrrhizin,glycyrrhizic acid and 6-gingerol were all greater than 1.5;the RSDs of the precision test were 0.74%,0.65%,and 0.69% respectively;the RSDs of the repeatability test were 1.1%,0.86%,0.92%;the RSDs of the stability test were 0.43%,0.78%,and 3.0%;the recoveries were 99.7%,104.5%,and 95.1%,respectively. In the fingerprint of the corresponding physical product,glycyrrhizin was used as the reference peak,and 15 common peaks were determined. By comparison with the reference substance,it was identified that peak 4 was glycyrrhizin,peak 13 was glycyrrhizin, and peak 14 was 6-ginger. The similarity between the corresponding physical fingerprint and the control fingerprint was good,and both were greater than 0.9. The average content of glycyrrhizin,glycyrrhizic acid, and 6-gingerol in 15 batches of material standards was 24.5,35.6,and 1.6 mg·g -1 ,respectively. Conclusion: The established fingerprint of Ganjianglingshu decoction and the content determination method for detecting the three components are simple,accurate and reliable,and can be used for quality control of its material standards.
LIU He, HE Dan-tong, XING Chun-lai, HE Rui, JIA Ai-ling, YANG Jing-yao, LIU Rui, QIU Zhi-dong, WEI Hong
. Establishment of HPLC fingerprint and content determination method of Ganjianglingzhu decoction *[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(1)
: 42
-50
.
DOI: 10.16155/j.0254-1793.2021.01.05
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