Metabolism Analysis

Analysis of metabolites in primary dysmenorrhea rats plasma, bile,urine and feces after administration of Guizhi Fuling capsules *

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  • 1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Key Laboratory for High Technology Research of TCM Formulae,Nanjing University of Chinese Medicine,Nanjing 210023,China;
    2. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine,Nanjing 210023,China;
    3. Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China

Received date: 2020-01-12

  Online published: 2024-05-31

Abstract

Objective: To determine the metabolites in primary dysmenorrhea rats after administration of Guizhi Fuling capsules. Methods: The ultra-high performance liquid chromatography-LTQ Orbitrap mass spectrometric (UHPLC-LTQ-Orbitrap-MS) and mass defect filter techniques with electrospray ion (ESI) source were applied to collect multi-level mass spectrometry fragment information in both positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C18 column (100 mm×2.1 mm,1.7 μm) with mobile phase of acetonitrile-water (containing 0.1% formic acid) by gradient elution at a flow rate of 0.4 mL·min -1 . After the administration of Guizhi Fuling capsules,the plasma,bile,urine and feces in rats were analyzed,and the metabolites were identified by mass loss filtration (MDF). Results: 8 prototypes and 50 metabolites were determined. The in vivo main metabolic reactions were phase Ⅰ reaction (decarboxylation, hydrolysis,dehydroxylation and dehydration) and phase Ⅱ reaction (methylation,acetylation,sulfonation and glucuronidation). Conclusion: LC-MS isused to identify metabolites of Guizhi Fuling capsules in rats with primary dysmenorrhea,and this study provids a basis for pharmacokinetic studies of the compound preparation of traditional Chinese medicine.

Cite this article

ZHAO Qiu-long, BIAN Xiao-kun, QIAN Da-wei, SU Shu-lan, GUO Sheng, WANG Tuan-jie, DUAN Jin-ao . Analysis of metabolites in primary dysmenorrhea rats plasma, bile,urine and feces after administration of Guizhi Fuling capsules *[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(1) : 51 -63 . DOI: 10.16155/j.0254-1793.2021.01.06

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