Objective: To establish a quantitative method for the determination of scutebarbatine A,scutebarbatine B,scutebata C and scutebata F in Scutellariae Barbatae Herba by UPLC-MS/MS. Methods: The determination was performed on an ACQUITY UPLC® BEH C18（2.1 mm×100 mm,1.7 μm）at 30 ℃ . The mobile phase of 0.1% formic acid solution（A）-0.1% formic acid acetonitrile（B）was subjected to gradient elution（0-7 min, 48%B → 100%B;7-8 min,100%B;8-8.5 min,100%B → 48%B;8.5-10 min,48%B）at the flow rate of 0.2 mL· min-1. The analytes were determined by electrospray ionization source（ESI）combined with multiple reaction monitoring （MRM）in positive ion mode. Results: Scutebarbatine A showed a good linearity in the range of 0.334 4- 13.38 μg· mL-1（r>0.999）,scutebarbatine B showed a good linearity in the range of 0.223 1-8.923 μg·mL-1（r>0.999）, scutebata C showed a good linearity in the range of 0.162 4-6.496 μg·mL-1（r>0.999）,and scutebata F showed a good linearity in the range of 0.198 8-7.954 μg·mL-1（r>0.999）. The average recoveries（n=9）were 98.2%, 98.6%,99.9% and 93.3%,and the corresponding RSDs were 4.2%,6.2%,4.6% and 6.3%,respectively. The contents of scutebarbatine A,scutebarbatine B,scutebata C and scutebata F in 27 batches of samples were 15.44- 168.4,14.98-118.23,13.31-62.40,12.37-117.67 μg·g-1,respectively. Conclusion: The method is competent in the quantification of the main diterpenoid nicotinates in Scutellariae Barbatae Herba.
YANG Guo-chun, L Xing, LIN Zi-wei, SUN Li-xin
. Simultaneous determination of four diterpenoid nicotinates in Scutellariae Barbatae Herba by UPLC-MS/MS*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(1)
: 130
-137
.
DOI: 10.16155/j.0254-1793.2021.01.16
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