Objective:To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of salidroside,nuezhenoside,specnuezhenide,oleuropein,luteolin, wedelolactone,apigenin and oleanolic acid in Erzhi pills(Ligustrum lucidum Ait.,Eclipta prostrata L.). Methods:An Agilent ZOBAX C18(250 mm×4.6 mm,5 μm)column was used in the HPLC assay. Acetonitrile-0.2% phosphoric acid aqueous solution with gradient elution was employed as the mobile phase.The flow rate was 1 mL·min-1,the column temperature was maintained at 30 ℃ and the detection wavelengths were 210,224 and 349 nm. Specnuezhenide was used as the internal reference substance to calculate the relative correction factors of the other seven constituents and evaluate their contents accordingly. Moreover,the external standard method(ESM) was used to quantify the eight components in Erzhi pills. Comparison of the difference between the QAMS and ESM was made. Results:In certain linear range,relative correction factors of salidroside,nuezhenoside,oleuropein, luteolin,wedelolactone,apigenin and oleanolic acid with reference to specnuezhenide were 0.716 7,1.136 2,1.261 3,3.917 6,1.917 4,4.194 6 and 0.557 9. The results were repeatable under different conditions with RSDs less than 3%. The contents of salidroside,nuezhenoside,specnuezhenide,oleuropein,luteolin,wedelolactone, apigenin and oleanolic acid in 8 batches of samples were 3.818-6.279 mg·g-1,2.214-4.062 mg·g-1,24.502-38.906 mg·g-1,0.252-0.539 mg·g-1,0.041-0.082 mg·g-1,0.036-0.132 mg·g-1,0.012-0.031 mg·g-1 and 4.352-8.104 mg·g-1 respectively by QAMS. There was no significant difference between the QAMS and the ESM. Conclusion:The established QAMS can quantify eight components in Erzhi pills accurately and reliably. This developed method can be used to determine multiple components in Erzhi pills.
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