Objective：To establish a method for the simultaneous determination of chlorogenic acid, paeoniflorin, naringin, baicalin, baicalein and wogonin in Qiguanyan pills. Methods：HPLC analysis was performed on the column of Agilent 5 TC-C18（2）（250 mm×4.6 mm, 5 μm）with the mobile phase of acetonitrile-0.2% phosphoric acid at a flow rate of 1.0 mL·min-1, the column temperature was 30 ℃, and the detection wavelength was 327 nm for chlorogenic acid, 230 nm for paeoniflorin and 280 nm for naringin, baicalin, baicalein, wogonin, and the volume injection was 10 μL. Results：Six active components showed good linear relationships within their own ranges（r>0.999）, the average recoveries were 101.0%（RSD=1.3%）, 99.2%（RSD=1.1%）, 98.3%（RSD=1.4%）, 99.9%（RSD=1.3%）, 101.2%（RSD=1.3%）and 101.5%（RSD=1.3%）respectively. The precision, stability, and repeatability were good with RSDs less than 2%. The content determination results of chlorogenic acid, paeoniflorin, naringin, baicalin, baicalein and wogonin were 0.295 9-0.375 8, 0.711 4-0.818 4, 0.361 4-0.489 2, 1.169-1.555, 0.035 76-0.052 87, 0.102 1-0.167 5 mg·g-1, respectively. Conclusion：The established method is simple, rapid, accurate and can be used for improvement of subsequent quality standards of Qiguanyan pills.
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