Objective：To establish a method for the specific detection of Liriopes Radix in Xiaokeling tablets. Methods：The samples were extracted by methanol-ammonia,rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry（RRLC-QQQ/MS）was used to detect Liriopes Radix． The Thermo AcclaimTMRSLC 120 C18（2.1 mm×100 mm,1.8 μm）column was used．Acetonitrile -20 mmol·L-1ammonium acetate solution was used as mobile phase,and gradient elution（0~20 min,5%A → 90%A）,flow rate was 0.2 mL·min-1．Column temperature was 30 ℃,injection was 2 μL．The ionized mode was ESI+,and m/z 723.4 → 251.2 and m/z 723.4 → 269.2 were selected as the ion pairs of liriopeside B,and m/z 893.5 → 463.1 and m/z 893.5 → 481.2 as the ion pairs of Liriope muscari Baily saponin C,for multi-reaction monitoring. Results：The detection limits of liriopeside B and Liriope muscari Baily saponin C were 0.28 μg per tablet and 1.50 μg per tablet, respectively．6 batches of samples from batch 111 Xiaokeling tablets were mixed with Liriope spicata var．prolifera Y．T．Ma and Liriope muscari（Decne．）Bailey,4 batches of samples were adulterated with Liriope spicata var． prolifera Y．T．Ma,and 15 batches of samples were adulterated with Liriope muscari（Decne．）Bailey,involving 25 batches of samples from 8 enterprises. Conclusion：The established method is accurate,rapid and simple,which can provide strong technical support for the special treatment of Xiaokeling tablets．
HUANG Bo, YAO Li, ZHANG Hui, ZHANG Ying-ting, LIN Yan-xiang, LUO Yi
. Rapid test of Liropes Radix in Xiaokeling tables by rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(2)
: 320
-328
.
DOI: 10.16155/j.0254-1793.2021.02.16
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