Objective：To establish the HPLC fingerprint of substance benchmarks of the classical famous prescriptions Baoyuan decoction in combination with chemical pattern recognition,establish determination methods of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and calycosin-7-O-glucoside in substance benchmarks,investigate the value transmitting relationship of medicinal herbs -decoction pieces-substance benchmarks of Panax Ginseng and Astragali Radix,and evaluate the rationality of preparation process of substance benchmarks by combining with transfer rates of effective components,so as to provide reference for its quality control. Methods：15 batches of substance benchmarks（samples）were prepared according to the method recorded in ancient books. HPLC fingerprint of 15 batches of test solution was establish. Similarity analysis was performed by using the Chinese medicine chromatographic fingerprint similarity evaluation system（2012A edition）. Mev-4.9.0 and SIMCA 14.1 software were used to conduct cluster analysis（CA）, principal component analysis（PCA）,orthogonal partial least squares discriminant analysis（OPLS-DA）and other chemical pattern recognition samples. The contents of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 and calycosin-7-O-glucoside were determined by HPLC. The transfer relation of quantity value was analyzed by effective component transfer rate. Results：Research on 15 batches of samples,HPLC fingerprint was established under 254 nm and 203 nm and the similarities were greater than 0.9. 27 common peaks were identified and 5 chromatographic peaks were identified at 254 nm（calycosin-7-O-glucoside,glycyrrhizin, cinnamic alcohol,cinnamic acid and ammonium glycyrrhIzinate）,22 common peaks were identified and 4 chromatographic peaks were identified at 203 nm（ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and 6-gingerol）. The test solution was stable within 48 h. The method showed good precision and repeatability. Three chemical pattern recognition analysis methods divided the substance benchmarks into three categories. 14 main components that caused the quality difference between batches were screened out. The average transfer rates of summation of ginsenosides Rg1 and Re,ginsenoside Rb1 and calycosin-7-O-glucoside from medicinal herbs to decoction pieces were 103.67%,102.68% and 110.30%,respectively,summation of ginsenosides Rg1 and Re,ginsenoside Rb1 and calycosin-7-O-glucoside from decoction pieces to substance benchmarks were 65.03%,68.44% and 59.77%,respectively. Conclusion：The methods of the HPLC fingerprint and content determination of substance benchmarks are simple and stable. The quality control of substance benchmarks of Baoyuan decoction based on chemical pattern recognition technology and index component content transfer rate can be evaluated systematically and comprehensively.
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