Objective: To establish HPLC characteristic chromatograms of Cistanche deserticola and Cistanche sinensis and to identify different Cistanche speices, so as to provide references for the identification and quality evaluation of the herb. Methods: HPLC analysis was performed on an Agilent Zobarx C18 column（250 mm×4. 6 mm, 5 μm）. The mobile phase consisted of acetonitrile（A）and 0. 1% formic acid aqueous solution（B）with gradient elution. The flow rate was 1 mL·min-1, the detection wavelength was 237 nm and the column temperature was 35 ℃. TCM chromatographic fingerprint similarity evaluation software（2012 version）was used for similarity analysis of four cistanche speices. Results: The chemical components of sinensis were different from those of C. deserticola, C. tubulosa and C. salsa. Among them, 10 common peaks of C. deserticola, C. tubulosa and C. salsa were marked, and 7 of them were identified. Ten common peaks of C. sinensis were marked and 5 of them were identified. By similarity evaluation software, the similarities of four Cistanche speices were compared. There were obvious differences between the characteristic chromatograms of C. deserticola and C. sinensis. There was no significant difference among the similarities of C. deserticola, C. tubulosa and C. salsa. On this basis, the peak area ratio of cistanoside A and echinacoside, and the peak area ratio of 2'-acetylacteoside and acteoside can be used as reference indexes to distinguish C. deserticola and C. tubulosa. Conclusion: The new method is suitable for the identification of C. deserticola, C. tubulosa and C. sinensis with high efficiency specificity. It provides a scientific basis for the identification and quality evaluation of them.
WANG Jia, YANG Wen, WANG Dong, ZHANG Xin-hua, GAO Lei
. Comparative studies of four Cistanche speices based on HPLC characteristic chromatogram[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(3)
: 384
-393
.
DOI: 10.16155/j.0254-1793.2021.03.02
[1] 朱晓玉, 刘雄, 高建德, 等. 肉苁蓉资源概况及药理作用新进展[J]. 甘肃中医药大学学报, 2016, 33(4): 91
ZHU XY, LIU X, GAO JD, et al. Resources of Cistanche deserticola and new progress of its pharmacological action[J]. J Gansu Univ Tradit Chin Med, 2016, 33(4): 91
[2] 石婷婷, 葛卫红. 肉苁蓉属植物的研究进展[J]. 中国民族民间医药, 2009, 18(9): 7
SHI TT, GE WH. The research development of Cistanche Hoffmg. et Link[J]. Chin J Ethnomed Ethnopharm, 2009, 18(9): 7
[3] 中华人民共和国药典2015 年版. 一部[S]. 2015: 135ChP 2015. Vol Ⅰ[S]. 2015: 135
[4] MORIKAWA T, XIE H H, PAN YN, et al. A review of biologically active natural products from a desert plant Cistanche tubulosa[J]. Chem Pharm Bull, 2019, 67: 675
[5] FU Z, FAN X, WANG X, et al. Cistanches herba: an overview of its chemistry, pharmacology, and pharmacokinetics property[J]. J Ethnopharmacol, 2017(219): 233
[6] LI Z M, LIN HN, GU L, et al. Herba Cistanche(Rou Cong-Rong): One of the best pharmaceutical gifts of traditional Chinese medicine[J]. Front Pharmacol, 2016, 7: 41
[7] BOUGANDOURA A, D’ABROSCA B, AMEDDAH S, et al. Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf. (Orobanchaceae) extract[J]. Fitoterapia, 2016, 109: 248
[8] 刘晓明, 姜勇, 孙永强, 等. 肉苁蓉化学成分研究[J]. 中国药学杂志, 2011, 46(14): 1053-1058.
LIU XM, JIANG Y, SUN YQ, et al. Study on chemical constituents of Cistanche deserticola[J]. Chin Pharm J, 2011, 46(14): 1053
[9] TU PF, SHI HM, SONG ZH, et al. Chemical constituents of Cistanche sinensis[J]. J Asian Nat Prod Res, 2007, 9: 79
[10] 龚华梦, 胡辉, 刘源才. HPLC 测定肉苁蓉配方颗粒的含量及指纹图谱研究[J]. 华西药学杂志, 2018, 33(6): 513
GONG HM, HU H, LIU YC. Study on the fingerprints and content determination of Cistanches herba dispensing granules by HPLC[J]. West Chin J Pharm Sci, 2018, 33(6): 513
[11] 李瑞燕, 赵明波, 屠鹏飞, 等. 一标多测法同时测定肉苁蓉中5 种苯乙醇苷类成分的含量[J]. 中国药(英文), 2019, 28(8): 537
LI RY, ZHAO MB, TU PF, et al. Simultaneous determination of five phenylethanoid glycosides in Cistanches Herba using quantitative analysis of multi-components by single marker[J]. J Chin Pharm Sci, 2019, 28(8): 537
[12] 路颖慧, 皮雯敏, 张悦, 等. 肉苁蓉和管花肉苁蓉的比较研究及市场现状分析[J]. 中国现代中药, 2019, 21(8): 999
LU YH, PI WM, ZHANG Y, et al. Comparative study and analysis of market status of Cistanche deserticoLa Y. C. Ma and Cistanche tubulosa(Schenk) Wight[J]. Modern Chin Medicine, 2019, 21(8): 999
[13] 马志国. 沙苁蓉与肉苁蓉饮片HPLC 指纹图谱鉴别研究[J]. 中国药学杂志, 2011, 46(12): 899
MA ZG. Differentiation of processed Cistanche sinensis G. Beck and herba Cistanches by HPLC fingerprints[J]. Chin Pharm J, 2011, 46(12): 899
[14] 杨建华, 胡君萍, 热娜·卡斯木, 等. 盐生肉苁蓉栽培品中苯乙醇苷类的指纹图谱研究[J]. 中国药学杂志, 2009, 44(15): 1128
YANG JH, HU JP, RENA KSM, et al. Studies on fingerprint of phenylethanoid glycosides in cultivated Cistanche salsa[J]. Chin Pharm J, 2009, 44(15): 1128
[15] 朱乃亮, 徐荣, 吴海峰, 等. 荒漠肉苁蓉和管花肉苁蓉指纹图谱比较研究[J]. 中国药学杂志, 2016, 51(13): 1116
ZHU NL, XU R, WU HF, et al. Fingerprint comparative analysis of Cistanche deserticola Y. C. Ma and Cistanche tubulosa(Schrenk)wight[J]. Chin Pharm J, 2016, 51(13): 1116
[16] 高妍, 过立农, 马双成, 等. 基于UPLC 特征图谱及主要成分含量的3 种肉苁蓉比较研究[J]. 中国中药杂志, 2019, 44(17): 3749
GAO Y, GUO LN, MA SC, et al. Comparative studies of three Cistanche speices based on UPLC specific chromatogram and determination of main components[J]. China J Chin Med, 2019, 44(17): 3749
