Objective: To evaluate autoimmune hepatitis（AIH）mice model induced by concanavalin A（ConA） through determination of metabolic profile of bile acids using LC-MS/MS. Methods: The AIH mice models were successfully induced by 30 mg·kg-1 ConA（model group）. The biochemical and pathological mechanisms were not changed in the models induced by 20 mg·kg-1 ConA（pre-model group）. Results: By analysis of serum bile acids profile, a part of the components in bile acids increased in pre-model group, and all of the components in bile acids increased significantly. By using the principle components analysis（PCA）and orthogonal projections to latent structures discriminant analysis（OPLS-DA）, bile acids profile could differentiate pre-model group mice from control group mice, and could screen the biomarkers which could pre-identification the Sub-diseases. Conclusion: This work can provide the base for study of the early clinical diagnosis and grading the course of disease in the patients with AIH.
RAO Zhi, WANG Li-jun, ZHANG Guo-qiang, JIN Yong-wen, WU Xin-an, QIN Hong-yan, WEI Yu-hui
. Pre-identification of autoimmune hepatitis mice model by bile acids profiles[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(3)
: 436
-445
.
DOI: 10.16155/j.0254-1793.2021.03.08
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