Objective: To analyze the optimum range of initial adhesive force, stripping degree and suitable drug loading of Sichuan Wu gel patch. Methods: Eight kinds of gel patch from different manufacturers and grades of quality were randomLy selected for quality reevaluation from four aspects: perception, initial adhesion, adhesion and peel. Single factor experiment and mixing the material design experiment was conducted to screen the base 240 mg, 480 mg and 960 mg were prepared respectively;48 Kunming clean-grade mice were used as the research subjects were randomLy divided into 6 groups, 8 in each group, and were given blank gel patch（model group）, indomethacin gel patch（positive group）and 120 mg（experiment A）and 240 mg（experiment B）Group）, 480 mg （experiment C）, 960 mg(experiment D） of Chuanwu gel patch, and the optimal dose of Chuanwu was evaluated by the mouse acetic acid writhing experiment. Results: The re-evaluation of the quality of the gel patch of the eight brands was quite different. Overall, the greater the initial adhesion, the holding force and the peeling degree, the worse the sensory evaluation of the gel patch;according to the test results, the optimal formula of the gel patch is: sodium polyacrylate 3%, sodium carboxymethyl cellulose 1. 5%, diatomaceous earth 4%, glycerin 25%. It could be seen from the mouse acetic acid writhing experiment that when the drug loading of each chuanwu gel patch was 480 mg and 960 mg, the shorter the incubation period of the mouse writhing, the smaller the number of writhing, the higher the analgesic rate, taking into account safety and other factors, it was believed that Chuanwu gel patch with a drug loading of 480 mg was more suitable for clinical application. Conclusion: The preparation of Chuanwu gel patch should not only ensure its analgesic and anti-inflammatory effects, but also give full consideration to its appearance, follow-up, placardability, safety and other characteristics, and control its initial adhesion, peeling degree, and drug loading within an appropriate range to promote its efficient and reasonable application in the clinic.
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