Objective: To determine the cytokine levels in immunity evaluation of dental restoration membrane of xenogeneic acellular dermal matrix using cytometric beads array（CBA）. Methods: The dental restoration membrane of xenogeneic acellular dermal matrix was selected as a test sample and implanted into Balb/C mice IL-6, IL-10, IL-17A, IL-23, TNF-α, IFN-γ, IFN-β, MCP-1 and GM-CSF were measured by CBA. The data of the test group and the control group were analyzed statistically. Results: Compared with the negative control group, there was no significant difference in the IL-10、IL-23、IFN-β content in the positive control group, while the IL-1α, IL-1β, IL-6, IL-17A, TNF-α, IFN-γ, MCP-1 and GM-CSF contents were significantly higher than the sham control group（P<0. 01）. In the sample groups, except for the high dose group of MCP-1 and TNF-α, which was significantly higher than the sham operation group（P<0. 01 and P<0. 05, respectively）. The contents of IL-6, IL-10, IL-17A, IL-23, TNF-α, IFN-γ, IFN-β, MCP-1, GM-CSF were not changed significantly（P>0. 05）. Conclusion: The dental restoration membrane of xenogeneic acellular dermal matrix have no significant effect on cytokines, as show at the levels of cytokines measured using CBA method.
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