Objective: To provide reference for quality control and authenticity identification of Arnebiae Radix medicinal materials and decoction pieces in the market. By studied on the ITS2 sequences’ characters of imported Arnebiae Radix, based on DNA barcoding and PCR-RFLP technologies. Methods: The ITS2 region was selected as the DNA barcode sequence for comparison and identification of imported Arnebiae Radix and reference medicinal materials. The ITS2 sequences of imported Arnebiae Radix from different sources with reference medicinal materials were compared based on DNA barcoding and PCR-RFLP technologies. Results: After the restriction endonucliase AluI enzyme digestion, the agarose-gel electrophoresis results of 39 imported Arnebiae Radix samples showed that, only DH3 had bands at around 500 bp, and none bands between 100 bp and 300 bp. And the results of other imported Arnebiae Radix samples had two or three obvious bands between 100 bp and 300 bp. The ITS2 sequences of imported Arnebiae Radix samples were compared with the reference medicinal materials, among which DH3 had the largest differences of 15 bases compared to the reference medicinal materials, the ITS2 sequence of F2 was same to the reference medicinal materials, and other imported Arnebiae Radix samples had 1-9 bases difference compared to the reference medicinal materials. The clustering results showed that the imported Arnebiae Radix sample DH3 was clearly distinguished from other imported Arnebiae Radix samples and reference medicinal materials which was in a single branch. There were 14 samples, which were clustered together with the reference medicinal materials in one branch with support rate ≥50%. Conclusion: The ITS2 region is selected to compare the similarities and differences of ITS2 sequences between imported Arnebiae Radix samples and reference medicinal materials based on DNA barcode and PCR-RFLP technologies, which provids a reference for effective identification of Arnebiae Radix medicinal materials and decoction pieces, and a strong guarantee for market supervision of Arnebiae Radix medicinal materials.
LIU Jie, DAI Sheng-yun, GU Hai-yuan, QIAO Fei, LIAN Chao-jie, GUO Li-nong, ZHENG Jian, MA Shuang-cheng, MI Jia
. The ITS2 sequences’ characters study of imported Arnebiae Radix based on DNA barcoding and PCR-RFLP technologies*[J]. Chinese Journal of Pharmaceutical Analysis, 2024
, 44(5)
: 750
-755
.
DOI: 10.16155/j.0254-1793.2024.05.02
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