Objective: To compare the quality of wild and cultivated Arnebiae Radix,using macroscopic investigation and chemometric analysis of the different components in wild and cultivated Arnebiae Radix from three different habitats. Methods: Wild and cultivated Arnebiae Radix were collected and their macroscopic features were compared. Using the ACQUITY UPLC BEH C18 (2.1 mm×100 mm, 1.7 μm) column, with acetonitrile-0.05% formic acid water as the mobile phase, the contents of D-shikonin, acetylshikonin, β-acetoxyisovalerylshikonin, isobutyrylshikonin, β,β’-dimethylacrylalkannin and isovalerylshikonin in 48 batches of wild and cultivated Arnebiae Radix were determined. The detection wavelength was 275 nm and the flow rate was 0.2 mL·min-1. PCA and OPLS-DA were performed to reveal the differential components of wild and cultivated Arnebiae Radix. Results: There were great differences in macroscopic features of wild and cultivated Arnebiae Radix, and the linear relationship between the contents of six naphthoquinone components was good. The correlation coefficients were above 0.999, the average recovery rates were 93.4%-102.9%, and the RSDs were less than 3.0%. The contents of six components in different batches of wild and cultivated Arnebiae Radix were quite different, and the contents of D-shikonin and acetylshikonin in wild products were significantly higher than those in cultivated products, indicating that there were still certain differences between wild products and cultivated products. The PCA model established could distinguish wild products and cultivars, and two differentiating components in wild products and cultivars were revealed by OPLS-DA, namely isobutyryl shikonin, β,β’-dimethylacrylalkannin. Conclusion: By comparing the core size, cork curl degree and specific odor of wild and cultivated products, the two can be identified. The established content determination method is repeatable, specific, stable and feasible. The differential components in wild and cultivated Arnebiae Radix in three different regions are identified, which provides a basis for the quality control of Arnebiae Radix and provides ideas for expanding the source of Arnebiae Radix.
HUANG Rui, DAI Sheng-yun, WU Dong-xue, MA Xiao-jun, LIU Jie, GUO Li-nong, Dao-er-jia-la, JING Song, MA Shuang-cheng, ZHENG Jian
. Comparative study on the quality of wild and cultivated Arnebiae Radix*[J]. Chinese Journal of Pharmaceutical Analysis, 2024
, 44(5)
: 783
-795
.
DOI: 10.16155/j.0254-1793.2024.05.06
[1] 佚名.神农本草经[M]. 北京:中国医药科技出版社,2018:99
YIMING. Shennong Bencaojing [M]. Beijing:China Medical Science Press, 2018:99
[2] 李时珍. 本草纲目[M]. 福州:福建科学技术出版社,2020:212
LI SZ. Bencaogangmu [M]. Fuzhou:Fujian Science & Technology Press, 2020:212
[3] 贾思勰. 齐民要术[M]. 北京:团结出版社,1996:467
JIA SX. Qimin Yaoshu [M]. Beijing:Unity Press,1996:467
[4] 中华人民共和国药典2020版,一部[S]. 2020:1902
ChP 2020. Vol Ⅰ [S]. 2020:1902
[5] 陈康,李敏.中药材种植技术[M] 北京:中国医药科技出版社,2006:443
CHEN K, LI M. Chinese Herbal Medicine Planting Technology[M]. Beijing:China Medical Science Press, 2006:443
[6] 徐海燕.新疆软紫草属资源及其药材品质评价的研究[D]. 北京:北京中医药大学,2021
XU HY. Research on the Quality Evaluation and Resources of Arnebia L. and Their Medicinal Materials [D]. Beijing:Beijing University of Chinese Medicine,2021
[7] 杨亮杰,刘秋琼,敬松,等.绿色食品原料新疆紫草栽培技术规程[J]. 现代农业科技,2020,766 (8):72
YANG LJ, LIU QQ, JING S, et al. Technical regulations for the cultivation of green food raw materials Arnebia euchroma [J]. Mod Agric Sci Technol, 2020, 766 (8):72
[8] 叶雨,樊丛照,张际昭,等. 基于MaxEnt和GIS的新疆紫草产地生态适宜性分析[J]. 中国现代中药,2022,24 (5):770
YE Y, FAN CZ, ZHANG JZ, et al. Ecological suitability of Arnebia euchroma producing area based on MaxEnt and GIS [J]. Mod Chin Med, 2022, 24 (5):770
[9] 丁文欢,王东东,王延蛟,等. 新疆3种软紫草资源现状调査与分析[J]. 时珍国医国药,2023,34 (1):162
DING WH, WANG DD, WANG YJ, et al. Investigation and analysis of three species of arnebia resource situation in Xinjiang [J]. Lishizhen Med Mater Med Res, 2023, 34 (1):162
[10] 钱雪,李海涛,曾万祥,等. 紫草化学成分、药理作用及产品应用研究进展[J]. 中国野生植物资源,2021,40 (3):52
QIAN X, LI HT, ZENG WX, et al. Research progress on chemical constituents,pharmacological effects and product application of gromwell root [J]. Chin Wild Plant Resour, 2021, 40 (3):52
[11] 徐景怡,吴硕,李玉环,等. 新疆紫草及其活性成分抗病毒作用研究进展[J]. 中国医药生物技术,2019,14 (6):549
XU JY, WU S, LI YH, et al. Research progress on the antiviral effect of Arnebia euchroma and its active ingredients [J]. Chin Med Biotechnol, 2019, 14 (6):549
[12] 廖梅,张声源. 新疆紫草的化学成分研究[J]. 中药材,2020,43 (11):2701
LIAO M,ZHANG SY. Chemical constituents from the roots of Arnebia euchroma [J]. J Chin Med Mater, 2020, 43 (11):2701
[13] 李静,李耀磊,于健东,等. 一测多评法结合特征图谱的新疆紫草质量控制研究[J]. 药物分析杂志,2020,40 (7):1209
LI J, LI YL, YU JD, et al. Quality control of Arnebiae Radix based on characteristic chromatogram and QAMS [J]. Chin J Pharm Anal, 2020, 40 (7):1209
[14] 昝珂,苏蕊,滕爱君,等. 新疆紫草HPLC特征图谱和紫草类药材萘醌类成分含量测定[J]. 药物分析杂志,2016,36 (9):1526
ZAN K, SU R, TENG AJ, et al. HPLC specific chromatogram of Arnebia euchroma and determination of six naphthoquinones in Boraginaceous Herbs [J]. Chin J Pharm Anal, 2016, 36 (9):1526
