Quality Control

Effect of different drying methods on the content of major active ingredients in fresh cutting products of Atractylodes chinensis*

Expand
  • College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China

Received date: 2023-04-18

  Online published: 2024-06-20

Abstract

Objective: To explore the effects of key operating units such as different water contents and drying methods in processing methods on the content of main active ingredients in Atractylodes chinensis (DC.) Koidz. fresh cutting slices. To develop an HPLC method for simultaneous determination of five active ingredient (atractylenolide Ⅲ, atractylenolideⅠ, atractylodin, β-eudesmol, atractylon) in Atractylodes chinensis. Methods: The Supersil ODS2 column (250 mm×4.6 mm, 5 μm ) was used, the mobile phase was 0.1% phosphoric acid (A)- methanol(B) with gradient elution, at the flow rate of 1.0 mL·min-1, the detection wavelength was 220, 270, 203 nm, and the column temperature was 30 ℃. The entropy weight-TOPSIS method and cluster analysis were combined to determine the best method of fresh cutting process of Atractylodes chinensis. Results: The validation results of the content determination methodology were good. The contents of five components(atractylenolide Ⅲ, atractylenolide Ⅰ, atractylodin, β-eudesmol, atractylon) were 0.052-0.243, 0.195-1.015, 2.022-4.418, 0.119-5.049, 0.209-8.638 mg·g-1, respectively. The optimal cutting moisture content range of Atractylodes chinensis fresh cutting was (40%±3%)-(50%±3%). The results of ANOVA showed that the contents of atractylenolide Ⅲ, atractylenin, β-eudesmol and atractylone were significantly higher in the seven processing(S1-S7) of fresh cutting than in the traditional raw-tanning group(S8). The entropy weight-TOPSIS analysis showed that 60 ℃ microwave drying, 40 ℃ microwave drying and 50 ℃ blast drying slices pieces have better quality. Cluster analysis resulted in three groups, accounting for 12.5%, 50% and 37.5% respectively. Conclusion: The results indicate that different drying methods and temperatures have a significant impact on the quality of Atractylodes chinensis slices. This study is valuable for the subsequent research in Atractylodes chinensis fresh cutting slices.

Cite this article

LU Meng-qi, ZHANG Xue-jing, XU Meng-dan, LIU Xiao-kang, GONG Ji-yu, ZHANG Tian-zhu . Effect of different drying methods on the content of major active ingredients in fresh cutting products of Atractylodes chinensis*[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44(5) : 874 -881 . DOI: 10.16155/j.0254-1793.2024.05.16

References

[1] XU K, YANG YN, FENG ZM, et al. Six new compounds from Atractylodes lancea and their hepatoprotective activities[J]. Bioorg Med Chem Lett, 2016, 26(21): 5187
[2] 邓爱平, 李颖, 吴志涛, 等. 苍术化学成分和药理的研究进展[J]. 中国中药杂志, 2016, 41(21): 3904
DENG AP, LI Y, WU ZT, et al. Advances in studies on chemical compositions of Atractylodes lancea and their biological activities[J]. China J Chin Mater Med, 2016, 41(21): 3904
[3] LIU QT, ZHANG SS, YANG XH, et al. Differentiation of essential oils in Atractylodes lancea and Atractylodes koreana by gas chromatography with mass spectrometry[J]. J Sep Sci, 2016, 39(24): 4773
[4] KIM HY, NAM SY, HUANG SY, et al. Atractylone, an active constituent of KMP6, attenuates allergic inflammation on allergic rhinitis in vitro and in vivo models[J]. Mol Immunol, 2016, 78: 121
[5] WEN Z, YOU XK, LIU B, et al. Formation of atractylone liposomes by rapid expansion from supercritical to surfactant solution[J]. Asia Pac J Chem Eng, 2011, 6(4): 624
[6] CHOI KH, JEONG SI, LEE JH, et al. Acetylene compound isolated from Atractylodes japonica stimulates the contractility of rat distal colon via inhibiting the nitrergic-purinergic relaxation[J]. J Ethnopharmacol, 2011, 134(1): 104
[7] JEONG SI, KIM SY, KIM SJ, et al. Antibacterial activity of phytochemicals isolated from Atractylodes japonica against methicillin-resistant staphylococcus aureus[J]. Molecules, 2010, 15(10): 7395
[8] HAN NR, MOON PD, NAM SY, et al. Inhibitory effects of atractylone on mast cell-mediated allergic reactions[J]. Chem Biol Interact, 2016, 258: 59
[9] SONG HP, LI RL, CHEN X. Atractylodes macrocephala Koidz. promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway[J]. J Ethnopharmacol, 2014, 152(1): 163
[10] 洪智慧, 杜伟锋, 李小宁, 等. 中药材产地趁鲜加工的可行性及相关建议[J]. 中华中医药杂志, 2021, 36(1): 80
HONG ZH, DU WF, LI XN, et al. Feasibility and suggestion about the fresh processing on origin of traditional Chinese medicinal materials[J]. J Tradit Chin Med, 2021, 36(1): 80
[11] 唐力英, 王祝举, 宋秉生, 等. 当归饮片趁鲜切制的可行性探讨[J]. 中国中药杂志, 2010, 35(23): 3150
TANG LY, WANG ZJ, SONG BS, et al. Study on feasibility of cutting process of freash Angelicae Sinensis Radix[J]. China J Chin Mater Med, 2010, 35(23): 3150
[12] 刘勇, 陈骏飞, 徐娜, 等. 趁鲜切制加工对三七药材干燥速率和质量的影响[J]. 中国中药杂志, 2019, 44(7): 1381
LIU Y, CHEN JF, XU N, et al. Effects of fresh-cut on drying rate and quality of Panax notoginseng[J]. China J Chin Mater Med, 2019, 44(7): 1381
[13] 于迪, 杨辛欣, 王莹, 等. 防风趁鲜切制的含水率及不同干燥方式对饮片质量的影响[J]. 中草药, 2022, 53(9): 2678
YU D, YANG XX, WANG Y, et al. Effect onquality of decoction pieces by different drying methods and moisture content of fresh Saposhnikoviae Radix cutting[J]. Chin Tradit Herb Drugs, 2022, 53(9): 2678
[14] 晏宇杭, 卢丽洁, 周永峰, 等. 川白芷产地趁鲜切制与传统切制方法对其质量的影响[J]. 中草药, 2021, 52(14): 4176
YAN YH, LU LJ, ZHOU YF, et al. Effect of fresh cutting and traditional cutting methods on quality of Angelica dahurica[J]. Chin Tradit Herb Drugs, 2021, 52(14): 4176
[15] 田甜, 纪玉华, 王寿富, 等. 车前草浸出物测定方法的建立及熵权TOPSIS法评价车前草药材质量[J]. 广东药科大学学报, 2021, 37(1): 16
TIAN T, JI YH, WANG SF, et al. Study on determination method of plantain extract and quality evaluation of plantain by entropy weight TOPSIS[J]. J Guangdong Pharm Univ, 2021, 37(1): 16
[16] 林永君, 白青飞. 基于组合权重与TOPSIS法的火力发电厂运行指标评价[J]. 山东电力技术, 2021, 48(8): 61
LIN YJ, BAI QF. Evaluation of thermal power plant operation index based on combined weight and TOPSIS method[J]. Shandong Electron Power, 2021, 48(8): 61
[17] 潘永康, 刘忠喜, 刘相东. 现代干燥技术[M]. 第二版. 北京: 化学工业出版社, 2007: 10
PAN YK, LIU ZX, LIU XD. Modern Drying Technology[M]. 2nd Ed. Beijing: Chemical Industry Press, 2007: 10
Outlines

/