Quality Control

Application of ginsenoside fingerprint in the quality control of Panax quinquefolius L. from Shandong*

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  • 1. Institute of Special Animal and Plant Sciences, China Academy of Agricultural Sciences, Changchun 130112, China;
    2. Agriculture and Rural Bureau of Wendeng District, Weihai City, Shandong Province, Weihai 264400, China

Received date: 2024-01-17

  Online published: 2024-06-21

Abstract

Objective: To establish the fingerprint of Panax quinquefolius L. from Shandong by UPLC-PDA, and to simultaneously determine the contents of 16 ginsenosides. Methods: The chromatographic column was an Acquity UPLC BEH C18 column(50 mm×2.1 mm, 1.7 μm), which was eluted with water-acetonitrile by gradient at a flow rate of 0.4 mL·min-1. The detection wavelength was 203 nm, the column temperature was 30 °C, and the injection volume was 2 μL. The Chinese Pharmacopoeia “Chinese Medicine Chromatography Fingerprint Similarity Evaluation System (2012 Edition)” was used for evaluation, and 42 batches of Panax quinquefolius L. from different habitats were compared with cluster analysis and principal component analysis. Results: The total content 16 ginsenosides in Panax quinquefolius L. from in Shandong were 19.73-58.07 mg·g-1, and the average value was (34.72±8.22) mg·g-1. The fingerprints of the ginsenosides in Panax quinquefolius L. from Shandong were established, and the similarities were above 0.90. And 10 common peaks constituted the characteristic peaks of Panax quinquefolius L.. Cluster analysis and principal component analysis showed that the contents of ginsenosides in Panax quinquefolius L. from Shandong were stable. Conclusion: The fingerprint of Panax quinquefolius L. established is highly characteristic, and the method issimple, which provides data support for the identification and quality control of Panax quinquefolius L..

Cite this article

ZHANG Yan-ting, LU Yu-shun, ZHANG Yue, REN Li-peng, WEI Xiao-ming, SUN Yin-shi . Application of ginsenoside fingerprint in the quality control of Panax quinquefolius L. from Shandong*[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44(3) : 512 -521 . DOI: 10.16155/j.0254-1793.2024.03.17

References

[1] 中华人民共和国药典2020年版. 一部[S]. 2020: 136
ChP 2020.Vol Ⅰ[S]. 2020: 136
[2] 丁涛,尚智,温富春,等. 西洋参茎叶总皂甙对小鼠腹腔巨噬细胞免疫功能作用的研究[J]. 长春中医药大学学报,2007,23(6): 14
DING T, SHANG Z, WEN FC, et al. Effects of total saponins from Panax quinquefolius L. stems and leaves on the immune function of mouse peritoneal macrophages[J]. J Changchun Univ Chin Med, 2007, 23(6): 14
[3] LI ZM, ZHAO LJ, CHEN JB, et al. Ginsenoside Rk1 alleviates LPS-induced depression-like behavior in mice by promoting BDNF and suppressing the neuroinflammatory response[J]. Biochem Biophys Res Commun, 2020, 530(4): 658
[4] KING L, MURPHY LL. Role of cyclin inhibitor protein p21 in the inhibition of HCT116 human colon cancer cell proliferation by American ginseng (Panax quinquefolius) and its constituents[J]. Phytomedicine, 2010, 17(3-4): 261
[5] WANG XX, WANG CM, WANG JM, et al. PseudoginsenosideF11(PF11) exerts anti-neuroinflammatory effects on LPS-activated microglial cells by inhibiting TLR4-mediated TAK1/IKK/NF-κB, MAPKs and Akt signaling pathways[J]. Neuropharmacology, 2014, 79: 642
[6] SHAO ZH, XIE JT, VANDEN HOEK TL, et al. Antioxidant effects of American ginseng berry extract in cardiomyocytes exposed to acute oxidant stress[J]. Biochim Biophys Acta, 2004, 1670(3): 165
[7] KIM MH, LEE J, JUNG S, et al. The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet[J]. J Ginseng Res, 2017, 41(2): 120
[8] 杨雄,楚世峰. 人参皂苷Rg1和Rb1在神经退行性疾病治疗中的作用[J]. 神经药理学报,2018,8(6): 56
YANG X, CHU SF. The role of ginsenosides Rg1 and Rb1 in the treatment of neurodegenerative diseases[J]. Acta Neuropharmacol, 2018, 8(6): 56
[9] 赵云利,吴华彰,杨晶,等. 西洋参皂甙对免疫抑制小鼠免疫功能的影响[J]. 中国生物制品学杂志,2011,24(3): 305
ZHAO YL, WU HZ, YANG J, et al. Effect of panax ginsenoside on immunological function of immuno-suppres-sive mice[J]. Chin J Biol, 2011, 24(3): 305
[10] 孙小单,王天鸣,李慧, 等. 人参皂苷Rh2抑制人非小细胞肺癌细胞增殖的机制研究[J]. 中草药,2022,53(2): 441
SUN XD, WANG TM, LI H, et al. Mechanism of ginsenoside Rh2 on inhibiting proliferation of human non-small cell lung cancer cells[J]. Chin Tradit Herb Drug, 2022, 53(2): 441
[11] 郑维维,张珊,唐彧,等. 基于CCL20/CCR6探讨人参皂苷Rh1调控乳腺癌细胞外渗的作用机制[J]. 中国药理学与毒理学杂志,2021,35(10): 756
ZHENG WW, ZHANG S, TANG Y, et al. The mechanism of ginsenoside Rh1 in regulating breast cancer cell extravasation based on CCL20/CCR6[J]. Chin J Pharmacol Toxicol, 2021, 35(10): 756
[12] TANG X, GAN XT, RAJAPUROHITAM V, et al. North American ginseng (Panax quinquefolius) suppresses β-adrenergic-dependent signalling, hypertrophy, and cardiac dysfunction[J]. Can J Physiol Pharm, 2016, 94(12): 1325
[13] 陆雨顺,张燕停,夏蕴实, 等. 不同形态鹿茸游离氨基酸的含量测定及质量评价应用研究[J]. 中国中药杂志, 2022, 47(6): 1587
LU YS, ZHANG YT, XIA YS, et al. Quality assessment of different forms of Cervi Cornu Pantotrichum based on content of free amino acids[J]. China J Chin Mater Med, 2022, 47(6): 1587
[14] 宋江峰,刘春泉,姜晓青,等.基于主成分与聚类分析的菜用大豆品质综合评价[J]. 食品科学,2015,36(13): 12
SONG JF, LIU CQ, JIANG XQ, et al. Comprehensive evaluation of vegetable soybean quality by principal component analysis and cluster analysis[J]. Food Sci, 2015, 36(13): 12
[15] 刘俊文,徐美利,徐冰, 等. 西洋参不同部位皂苷成分研究[J]. 天津中医药,2019,36(7):715
LIU JW, XU ML, XU B, et al. Study on the components of saponins in different parts of Panax quinquefolius L. [J]. Tianjin J Tradit Chin Med, 2019, 36(7): 715
[16] 陈丽雪,曲迪,华梅, 等. 不同年生和不同部位人参样品有效成分的比较[J]. 食品科学,2019,40(8): 124
CHEN LX, QU D, HUA M, et al. A comparative study of effective components in Panax ginseng C. A. Mey. samples from different parts and ages[J]. Food Sci, 2019, 40(8): 124
[17] 徐静,贾力,赵余庆. 人参的化学成分与人参产品的质量评价[J]. 药物评价研究,2011,34(3): 199
XU J, JIA L, ZHAO YQ. Constituents of Panax ginseng C. A. Mey. and quality evaluation of Panax ginseng C. A. Mey. products[J]. Drug Eval Res, 2011, 34(3): 199
[18] 尹春梅,李秀昌,孙佳明, 等. 西洋参中人参总皂苷含量的聚类分析[J]. 吉林农业大学学报, 2010, 32(3): 289
YIN CM, LI XC, SUN JM, et al. Cluster analysis of content of total saponin in Panax quinquefolius L. [J]. J Jilin Agric Univ, 2010, 32(3): 289
[19] 李嘉欣,李梦瑶,张单丽, 等. HPLC法同时测定不同生长年限、部位西洋参中2类成分[J]. 中成药,2020,42(10): 2670
LI JX, LI MY, ZHANG DL, et al. Simultaneous determination of two types of constituents in Panax quinquefolius L. with different growing years and parts by HPLC[J]. Chin Tradit Pat Med, 2020, 42(10): 2670
[20] 陈军辉,谢明勇,章志明, 等. 12种西洋参中总皂苷及人参皂苷Rb1的测定比较[J]. 时珍国医国药,2005(9): 845
CHEN JH, XIE MY, ZHANG ZM, et al. Determination and comparison of the contents of total ginsenoside and ginsenoside Rb1 in 12 kinds of Panax quinquefolius L. [J]. Lishizhen Med Mater Med Res, 2005(9): 845
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