Objective: To determine the polysaccharide hydrolysates from Polygonati Rhizoma under glycoside enzymatic hydrolysis' HPLC fingerprint. Examine the variations among the polysaccharides produced by the various varieties of Polygonati Rhizoma and provide references for the assessment of the polysaccharide quality. Methods: After the polysaccharide from Polygonati Rhizoma was hydrolyzed by fructose enzymes, its fingerprint was established by HPLC-ELSD. The fingerprint was then analyzed using similarity analysis (SA), hierarchical cluster analysis (HCA), and principal component analysis (PCA) to determine the differences between the polysaccharides from various Polygonati Rhizoma varieties. Results: Polysaccharides from various strains of Polygonati Rhizoma had different HPLC-ELSD fingerprints, and a total of 17 distinct oligosaccharide fragments were discovered, all of which contained fructose, glucose, and sucrose. Analysis revealed that there are significant intra species differences, minor differences, and high levels of similarity between the three types of Polygonati Rhizoma. The real Polygonati Rhizoma differs significantly from the imitation in several important ways. Conclusion: Polygonati Rhizoma can be successfully classified according to varieties, using the fingerprint of polysaccharide hydrolysates of the various varieties of Polygonati Rhizoma and its adulterants. The developed HPLC method can be used for the differential analysis of polysaccharides in Polygonati Rhizoma and is straightforward, precise, and repeatable.
DIAO Zhuo, HU Chong, YANG Qing-shan, ZHANG Ya-zhong
. Polygonati Rhizoma polysaccharide differential analysis based on enzymolysis and chemometrics*[J]. Chinese Journal of Pharmaceutical Analysis, 2024
, 44(2)
: 324
-332
.
DOI: 10.16155/j.0254-1793.2024.02.16
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