Ingredient Analysis

Evaluation of glucoamylase activity in Liushenqu based on HPLC determination of glucose content after derivatization*

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  • National Institutes for Food and Drug Control, Beijing 102629, China

Received date: 2021-05-24

  Online published: 2024-06-21

Abstract

Objective: To establish a pre-column derivatization high performance liquid chromatography method to determine the content of glucose for evaluation of glucoamylase activity, so as to evaluate the quality of the commercial Liushenqu. Methods: The sample was derivatized before the column with 1-phenyl-3-methyl-5-pyrazolone (PMP) as the derivatization reagent. HPLC analysis was performed on a C18 chromatographic column (250 mm×4.6 mm, 5 μm) with gradient elution of acetonitrile and 0.05 mol·L-1 phosphate buffer (pH 6.8). The column temperature was 30 ℃ and the detection wavelength was 250 nm. Results: The correlation coefficient of the linear equation of glucose concentration was 0.999 4 and the average recovery was 100.6% with RSD below 3.0%. The glucoamylase activities of different manufacturers ranged from 4.51 U·μmol-1 to 290.27 U·μmol-1. The result showed that the glucoamylase activity of different manufacturers varied greatly.Conclusion: This method can be used to determine the activity of glucoamylase in Liushenqu and provide data support and theoretical basis for the quality evaluation of Liushenqu.

Cite this article

WANG Jun-yao, CHENG Xian-long, LI Ming-hua, LI Ting, WEI Feng, MA Shuang-cheng . Evaluation of glucoamylase activity in Liushenqu based on HPLC determination of glucose content after derivatization*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(1) : 121 -126 . DOI: 10.16155/j.0254-1793.2022.01.13

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