Objective: To determine the contents of 7 active components(neoastilbin, astilbin, neoisoastilbin, isoastilbin, engeletin, isoengeletin, limonin) in the psoriasis external prescription. Methods: The column was Waters Xbrige BEH C18(150 mm×4.6 mm, 3.5 μm). The mobile phase consisted of solvent A(water) and solvent B(acetonitrile) with gradient elution (0-5 min, 17%B; 5-10 min, 17%B→20%B; 10-15 min, 20%B; 15-25 min, 20%B→100%B). The flow rate was 1.0 mL·min-1. The column temperature was 30 ℃. Injection volume was 5 μL. The detection wavelength for neoastilbin, astilbin, neoisoastilbin, isoastilbin, engeletin and isoengeletin were set at 292 nm during 0~19 min, and 210 nm for limonin during 19~25 min. MATLAB was used for two-wavelength fusion of CSV data. Results: The calibration curves of neoastilbin, astilbin, neoisoastilbin, isoastilbin, engeletin, isoengeletin and limonin were all in good linearity over the ranges of 39.06-1 250 μg·mL-1(r=0.999 9), 26.25-840 μg·mL-1(r=0.999 7), 18.64-596.4 μg·mL-1(r=0.999 8), 6.31-202 μg·mL-1(r=0.999 6), 2.53-80.8 μg·mL-1(r=0.999 8), 1.33-42.6 μg·mL-1(r=0.999 7) and 5.08-162.4 μg·mL-1(r=0.999 6), respectively, and the average recoveries(n=6) were 96.1%~102.2% with RSD of 0.48%-1.7%. The contents of neoastilbin, astilbin, neoisoastilbin, isoastilbin, engeletin, isoengeletin and limonin in samples self-made and provided by a hospital were 1.06, 1.21, 0.87, 0.30, 0.09, 0.07, 0.22 mg·mL-1 and 0.35, 0.32, 0.17, 0.08, 0.04, 0.03, 0.21 mg·mL-1, respectively. Conclusion: This established method can determine the contents of 7 active ingredients in psoriasis external prescription quickly and accurately, and is also helpful for the quality control of psoriasis external prescription.
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