Objective: To develop an HPLC method for simultaneous determination of three flavonoids (luteolin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide, apigenin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide and luteolin) and four phenolic acids (chlorogenic acid, cryptochlorogenic acid, caffeic acid and rosmarinic acid) in Glechomae Herba. Methods: The Waters SunFire C18 column(250 mm×4.6 mm,5 μm) was used, the mobile phase was acetonitrile(A)-0.1% phosphoric acid(B) with gradient elution, at the flow rate of 1.0 mL·min-1, the detection wavelength was 330 nm, and the column temperature was 30 ℃. Results: Seven constituents showed good linear relationships within their own ranges (r≥0.999 6), and the precision (RSD≤0.87%) and stability(RSD≤1.1%) were good. The average recoveries (n=6) were 99.1%-101.1% with the RSDs of 0.59%-1.7%. The contents of chlorogenic acid, cryptochlorogenic acid, caffeic acid, luteolin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide, apigenin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide, rosmarinic acid and luteolin in eighteen batches of samples were 0.046-0.602 mg·g-1, 0.071-0.817 mg·g-1, 0.102-1.159 mg·g-1, 0.728-4.817 mg·g-1, 0.592-4.081 mg·g-1, 0.850-13.060 mg·g-1 and 0.019-0.198 mg·g-1, respectively. Conclusion: This method is specific, accurate and reproducible, and can be used to control the quality of the Glechomae Herba.
LIAO Yuan-yue, YUAN Ming-ming, ZHOU Lei-gang, WU Xi, YU He-yun
. Simultaneous determination of three flavonoids and four phenolic acids in Glechomae Herba by HPLC*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(10)
: 1664
-1671
.
DOI: 10.16155/j.0254-1793.2023.10.04
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