Objective: To provide reference for the identification and quality evaluation of Guanhuaroucongrong formula particles, and to establish the method of fingerprint and the multi-component content determination by HPLC. Methods: The separation was performed on an Agilent Zorbax C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase. The flow rate was 1.0 mL·min-1, the detection wavelength was 238 nm, the column temperature was 30 ℃, and the injection volume was 10 μL. Results: The fingerprint was established with 12 common peaks. 6 characteristic peaks including geniposidic acid, caffeic acid, echinoside, acteoside, tubuloside A and iso-acteoside were identified. The content determination method of them was further established. The contents of the above 6 components in 7 batches of samples were respectively 0.57-2.22 mg·g-1, 0.19-0.41 mg·g-1, 29.36-52.99 mg·g-1, 1.64-4.99 mg·g-1, 2.09-3.97 mg·g-1 and 2.29-4.76 mg·g-1. Conclusion: The method of fingerprint and content determination of 6 components established in this study is feasible, which can provide scientific basis for comprehensive control and evaluation of the quality of Guanhuaroucongrong formula particles.
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