Process Evaluation

Analysis on the correlation between index composition and powder color during processing of carbonized ginger*

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  • Guangdong Yi Fang Pharmaceutical Co., Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China

Revised date: 2023-10-10

  Online published: 2024-06-21

Abstract

Objective: To study the correlation between HPLC fingerprint and chroma value of carbonized ginger during processing. Methods: Waters Symmetry (250 mm×4.6 mm, 5 μm) was used as the column, acetonitrile solution and water was used as the mobile phase by gradient elution. The flow rate was 1.0 mL·min-1. The chroma values (L*, a*, b*) of carbonized ginger powder at different processing times were determined by spectrophotometer. Similarity evaluation was carried out by Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine (2012). The clustering analysis (CA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were carried out with characteristic peak relative peak area and chromaticity value as indexes. SPSS 26.0 software was used to analyze the correlation between the chromaticity value of powder and the composition content and multiple step-up regression analysis, and the quantitative model between the chromaticity value and the chemical composition was established. Results: The similarity evaluation results showed that the similarity of carbonized ginger fingerprint decreased gradually with the extension of processing time. The unit peak area of the 10 characteristic peaks in HPLC fingerprints was correlated with the chroma value to varying degrees. The six components (compound 2, 6-gingerol, 8-gingerol, 10-gingerol, compound 9, and compound 10 ) that decreased with the deepening of carbonization degree were positively correlated with the chroma value. The contents of gingerone, 6-gingerenol, 8-gingerenol and 10-gingerenol were negatively correlated with chromaticity value. Stepwise regression analysis showed that the chromaticity value could predict the unit peak area of 6-gingerol, 8-gingerol and 10-gingerol to a certain extent. HCA divided the samples into three categories, and OPLS-DA screened three differential components with VIP values greater than 1. Conclusion: There is a certain correlation between the color of carbonized ginger powder and the unit peak area of characteristic peak, which can provide reference for the dynamic monitoring of chemical components of carbonized ginger in the process of processing.

Cite this article

CHEN Dan-yan, WANG Yu-ting, PAN Ling, HE Rong-rong, HUANG Sen, SUN Dong-mei, SHI Wen-ting . Analysis on the correlation between index composition and powder color during processing of carbonized ginger*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(10) : 1800 -1812 . DOI: 10.16155/j.0254-1793.2023.10.19

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