Objective: To establish the UPLC fingerprint of Taraxaci Herba, and to determine the contents of ten active ingredients (caffeic acid, rutin, umbelliferone, luteolin-7-O-glucoside, cichoric acid, quercetin, apigenin, kaempferol, diosmetin and genkwanin). Methods: The chromatographic separation was performed on a Waters T3 C18 column (100 mm×2.1 mm, 1.7 μm). Acetonitrile-0.4% orthophosphoric acid aqueous solution was used as mobile phase for gradient elution, and fingerprints of 15 batches of Taraxaci Herba were established by using traditional Chinese medicine chromatographic fingerprint similarity evaluation software. The quality of Taraxaci Herba was evaluated by clustering analysis (CA), principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and other stoichiometric methods. Results: The results showed that the UPLC fingerprint of Taraxaci Herba was established with 23 common peaks and 11 identified components. The content of 10 active components were determined (caffeic acid 0.11-0.60 mg·g-1, rutin 3.47-18.02 mg·g-1, umbelliferone 3.46-14.60 mg·g-1, luteolin-7-O-glucoside 0.23-0.32 mg·g-1, cichoric acid 6.98-8.68 mg·g-1, quercetin 2.19-7.17 mg·g-1, apigenin 0.29-1.03 mg·g-1, kaempferol 0.60-0.87 mg·g-1, diosmetin 0.17-1.25 mg·g-1, genkwanin 0.05-0.23 mg·g-1). The linear relationship of 10 components in their respective ranges was good (r≥0.999), and the average recoveries were in the range of 91.7%-96.3% (RSD<1.9%). Conclusion: Established UPLC fingerprint of Taraxaci Herba and the determination method for the 10 components are simple and easy with high precision and good repeatability, which provides a reference for the internal quality evaluation of Taraxaci Herba.
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