Objective: To establish an UPLC method for the analysis of eight active components Gentiana szechenyii Kanitz, Gentiana veitchiorum Hemsl, Gentiana lawrencei var. farreri and Comastoma pulmonarium, and to compare the differences of active components in the four Tibetan medicinal materials. Methods: A UPLC method was set up, an InfinifityLab Poroshell 120 SB-C8 (150 mm×2.1 mm,2.7 μm)chromatographic column was used, the mobile phase was acetonitrile (B)-0.05% acetic acid aqueous solution (C) with a flow rate of 0.2 mL·min-1, gradient elution, detection wavelength of 254 nm, and column temperature of 25 ℃. Results: The linear regression equation correlation coefficient of the eight components of loganic acid, swertiamarine, gentiopicroside, sweroside, isoorientin, isoscoparin-2″-O-glucopyranoside, isovitexin, isoscoparin was 0.999 8-1.000, the average recovery (n=6) was 98.4%-102.1%, and the RSD was 0.77%-2.3%. The total contents of 8 active components in 4 species of Gentianaceae plants were as below: the highest content was 85.63 mg·g-1 in Comastoma pulmonarium, followed by 66.01 mg·g-1 in Gentiana veitchiorum Hemsl, 42.11 mg·g-1 in P. Gentiana lawrencei var. farreri, and 19.33 mg·g-1 in Gentiana szechenyii Kanitz. All four gentianaceae Tibetan medicines contain loganic acid and isovitexin, but their contents were low. The contents of gentiopicroside and sweroside in Gentiana scabra were high, both exceeding 10 mg·g-1. The content of gentiopicroside in Gentiana rigescens was the highest of (19.26±1.67) mg·g-1, and they all did not contain isorkinin-2″-O-pyranoside. Swertiamarin, gentiopicroside. Sweroside were not detected in Gentiana macrophylla, but the contents of isoflorin-2″-O-pyranoside and isoflorin were higher, reaching (7.99±0.09) mg·g-1 and (6.77±0.03) mg·g-1, respectively. The highest content of swertiamarin was (76.79±7.42) mg·g-1, but gentiopicroside and isofraxidin were not detected. Conclusion: The established method is simple, accurate and can be used for the quality evaluation of the four gentianaceae Tibetan medicines. The content analysis results of eight active components in four gentianaceae Tibetan medicinal materials from Qinghai can provide reference for the in-depth study and utilization of their resources.
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