Objective:To establish the HPLC fingerprint of Vicatia thibetica de Boiss. and to compare its similar species to find out the commonalities and differences of the components among the species. Methods: The HPLC fingerprint method was established by collecting 26 representative batches of Vicatia thibetica de Boiss. samples from the main production area. The chromatography fingerprint similarity evaluation software(2012. 130723 version) was used to compare Vicatia thibetica de Boiss. and its similar species, including 14 batches of Sphallerocarpus gracilis (Bess.) K. -Pol., 4 batches of Pleurospermum hookeri var. thomsonii C. B. Clarke, 1 batch of Anthriscus. nemorosa (M. Bie.) Spreng., 6 batches of Ligusticum daucoides (Franch.) Franch., 1 batch of Vicatia coniifolia (Wall.) DC., 2 batchs of Anthriscus sylvestris (L.) Hoffm., 1 batch of Angelica acutiloba (Sieb. et Zucc.) Kitagawa, and 5 batchs of Pleurospermum franchetianum Hemsl. Meanwhile, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed on the three clinically commonly used Jiawa species, using SIMCA software with the quantified data of shared peak areas as variables. Results: Seventeen shared peaks were comfirmed, and seven shared peaks of L-tryptophan, chlorogenic acid, ferulic acid, isochlorogenic acid A, isochlorogenic acid C, chuanxiong lactone I and ligustrolactone were identified. The results of similarity evaluation and PCA and PLS-DA showed that there were significant differences among species, and six markers of inter-species differences were screened. Conclusion: The established HPLC fingerprint combined with multivariate statistical analysis can identify Vicatia thibetica de Boiss. and its similar species, and there is a large variability in the composition among the species. The seven shared peaks were identified as the main active ingredients, laying the foundation for the development of its quality standard.
WANG Yi-yu, XU Wei, QI Jing-liang, GAO Bi-xing, GOU Yan, LI Qian, GAO Chi, WANG Ying, YANG Lei, LAN Zhi-Qiong
. Comparative study on HPLC fingerprints of Vicatia thibetica de Boiss. and its similar species*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(11)
: 1925
-1934
.
DOI: 10.16155/j.0254-1793.2023.11.16
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