Quality Control

Evaluation of difference between fresh processing Cistanche and traditional processing Cistanche based on the internal quality and the stoichiometric methods*

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  • Gansu Provincial Institute of Drug Inspection and Research, National Key Laboratory of Quality Control of Chinese Medicinal Materials and Drinking Tablets, Gansu Provincial Engineering Laboratory of Inspection and Testing of Chinese and Tibetan Medicines, Lanzhou 730000, China

Revised date: 2023-08-04

  Online published: 2024-06-21

Abstract

Objective: To evaluate the difference between Cistanche fresh processing and Cistanche traditional processing by the combination of the internal quality and the stoichiometric methods, and to provide a reference for quality evaluation and determination of fresh processed Cistanche. Methods: Six internal quality control indexes, including water rate, total ash rate, extract rate, polysaccharide content, echinoside concent and creinoside concent, were determined in four processed samples. Cluster analysis (CA), principal component analysis (PCA-DA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were used to analyze the determination results. Results: The CA results showed that fresh-dried Cistanche and freeze-dried Cistanche were divided into one group, and Cistanche and wine-processed Cistanche were grouped separately. PCA results were similar to the CA. PLS-DA results showed that the clustering effect of the four kinds of processed Cistanche was good, but the fresh-dried Cistanche and freeze-dried Cistanche were concentrated, and the main difference indexes were polysac Charides concent, echinoside content, and extract rate. The OPLS-DA analysis of fresh and freeze-dried Cistanche showed that the main difference indexes were polysaccharide content, echinoside content and creinoside content. Conclusion: The quality of fresh processing Cistanche is different from that of traditional processing. The evaluation model of Cistanche is established by the combination of the internal quality and the stoichiometric methods, which can provide a reference for the feasibility and quality control of Cistanche fresh processing methods.

Cite this article

JIN Wan-jun, NI Lin, LIN Rui-li, LAI Jing, LIANG Yi-bo, LIU Fu-qiang, SONG Ping-shun . Evaluation of difference between fresh processing Cistanche and traditional processing Cistanche based on the internal quality and the stoichiometric methods*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(11) : 1935 -1945 . DOI: 10.16155/j.0254-1793.2023.11.17

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