Quality Control

Quality aanalysis of Marshmallow Flowers based on multi-component determination*

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  • 1. Inspection and Testing Center of Market Regulation Administration of Pinggu District, Beijing 101200, China;
    2. Xinjiang Institute of Chinese and Ethnic Medicine, Urumchi 830002, China;
    3. Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China

Received date: 2022-09-16

  Online published: 2024-06-21

Abstract

Objective: To optimize an HPLC method for determination of rutin, hypericin, isoquercitrin and quercetin in Marshmallow Flower. The contents of the above 4 components were taken as the index, the effects of methanol volume fraction, solid-liquid ratio and extraction time on the extraction efficiency was investigated by Box-Behnken response surface method, and the extraction process was optimized. Methods: HPLC was conducted on SB-C18 column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile and 0.2% phosphoric acid, with gradient elution at a flow rate of 1.0 mL·min-1. The column temperature was 35 ℃ and the detection wavelength was 284 nm. Based on single factor experiment,Box-Behnken response surface method was used to optimize the extraction process of Marshmallow Flower,using rutin, hypericin, isoquercitrin and quercetin as indicators. Results: Rutin, hypericin, isoquercitrin and quercetin exhibited good linearity (0.999 1-0.999 3) among the ranges of 5.025-50.25, 10.20-102.0, 19.91-199.1 and 16.85-168.5 μg·mL-1, respectively. The average recoveries were between 91.0%-96.2%.There were significant differences in component contents between different habitats and batches.The optimum process conditions for Marshmallow Flower were as follows: methanol volume fraction was 59%, solid-liquid ratio was 1∶50, extraction time was 90 min, and the predicted results were similar to the method verification results, and the method was feasible and reliable. Conclusion: The established content determination method was stable, reliable and reproducible, Model established by Box-Behnken response surface methodology has good predictability, which can be used for the quality evaluation and optimizing the extraction process of Marshmallow Flower.

Cite this article

LIU Hong-yu, SHI Lei-ling, MA Guo-xu, SUN Zhao-cui, YANG Jun-shan, XU Xu-dong, ZHANG Yue, FAN Cong-zhao . Quality aanalysis of Marshmallow Flowers based on multi-component determination*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(11) : 1955 -1964 . DOI: 10.16155/j.0254-1793.2023.11.19

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