Objective:To establish a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method for molecular identification of root of Liriope spicata. Methods: The universal primers for root of Liriope spicata, root of Liriope muscari and root of Ophiopogon japonicus were designed in ITS 2 region. After PCR reaction with the universal primers, the PCR products was digested by restriction endonuclease (Nae I) at 37 ℃ for 60 min. Results: Both root of Liriope spicata and root of Ophiopogon japonicus could be amplified by a single 377 bp band by the designed universal primers. The PCR chromatograms of root of Liriope spicata were cut into two bands, 231 bp and 146 bp after enzymatic digestion by Nae I, while root of Ophiopogon japonicus remained a single band of 377 bp, allowing molecular identification of root of Liriope spicata. Conclusion: Root of Liriope spicata can be identified accurately by the PCR-RFLP method. This method is simple, specific and stable, the identification results are objective and easy to interpret.
DUAN Qing- zi, WANG Wei, SHANG Ke, WEI Feng, MA Shuang-cheng, ZHANG Wen-juan
. A new method based on PCR-RFLP for identification of root of Liriope spicata*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(11)
: 1974
-1979
.
DOI: 10.16155/j.0254-1793.2023.11.21
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