Objective: To study the mechanism of Yinhuo Tang in improving depression like behavior in ovariectomized (OVX) combined with chronic unpredictable mild stress (CUMS) mice was explored by using the method of serum metabolomics. Methods: Forty-five Kunming mice were randomly divided into sham operation group, ovariectomy model group and Yinhuo Tang group. OVX+CUMS were used to replicate the postmenopausal depression (PMD) model. Open field test and forced swimming test were used to observe the depression like behavior of mice. HE staining was used to observe the state of hippocampal neurons in each group. Metabonomics method was used to observe the changes of serum differential metabolites in each group of mice, and its internal biological significance was analyzed. Results: The behavioral results showed that the depression like behavior of OVX+CUMS mice was significantly improved after administration of Yinhuo Tang. HE staining showed that the morphology of hippocampal neurons was normal. The results of metabonomics study showed that the metabolic profile of blood in mice after administration of Yinhuo Tang had a significant callback trend, and a total of 11 differential metabolites were identified, including citric acid, pinellic acid, sphingomyelin, sphingosine 1-phosphate, prostaglandin J2, alpha-dimorphecolic acid, (S)3-hydroxybutyric acid, PI[16∶0/22∶4 (10Z, 13Z, 16Z, 19Z)], p-cresol glucuronide, p-cresol sulfate and tyrosine, which had obvious callback effects. Conclusion: Yinhuo Tang can improve the depression like behavior of OVX+CUMS induced depression mice, and its mechanism may be achieved by regulating linoleic acid metabolism, citric acid cycle, sphingolipid metabolism, glutamate metabolism, glyoxylic acid and steroid hormone biosynthesis metabolic pathway.
XU Hong-dan, HU Jing, DING Ya-ming, ZHANG Yue, ZHANG Ning
. Metabonomics study on Yinhuo Tang improving depressive behavior in OVX+CUMS mice*[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(12)
: 2030
-2037
.
DOI: 10.16155/j.0254-1793.2023.12.06
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