Objective: To establish a Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to detect 4 androgens (nandrolone, 4-androstene-3,17-dione, testosterone and methylthstosterone), 3 progestins(19-norethindrone, progesterone and medroxyprogesterone), 4 estrogen(diethylstilbestrol, hexestrol, estradiol and estriol) and 2 glucocorticoids(cortisone and cortisol) in Cervi Cornu Pantotrichum slices. Contents of 13 hormones were determined in different parts of Cervi Cornu Pantotrichum (wax slices, powder slices, gauze slices and bone slices). Methods: Samples were extracted with methanol and purified by primary secondary amine (PSA) and Al2O3. The samples were separated by ACQUITY BEH C18 column(100 mm×2.1 mm, 1.7 μm) and detected by multiple reaction monitoring (MRM) in electrospray positive and negative ion mode respectively. Results: 4-androstene-3, 17-dione, testosterone, progesterone, diethylstilbestrol, hexestrol, estradiol, estriol, cortisone and cortisol were detected. Nandrolone, 19-norethindrone, methyltestosterone and medroxyprogesterone were not detected in Cervi Cornu Pantotrichum. The content of 4-androstene-3, 17-dione as androgens was the lowest (0.29 μg·kg-1) in 10 batches Cervi Cornu Pantotrichum, which was lower than testosterone (1.95 μg·kg-1). Four kinds of estrogen were relatively high, accounting for 50.7% of the 13 kinds of hormones, among which diethylstilbestrol was the highest (3.23 μg·kg-1). The contents of 13 hormones in wax slices, powder slices, gauze slices and bone slices decreased successively. The average content of 13 hormone was 34.62 μg·kg-1 in wax slices, which was higher than that in powder slices, gauze slices and bone slices (19.68, 14.60 and 12.72 μg·kg-1,respectively). Conclusion: The sensitivity, accuracy and precision of this method meet the detection requirements. It is suitable for the detection of hormones in Cervi Cornu Pantotrichum.
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