Objective: To develop a new ion chromatographic (IC-CD) method for the determination of nitrite in salt by using H2O2 oxidation couple with valve-switching. Methods: NO2- in samples was first oxidized to NO3-by H2O2,and then removed most Cl- and Br- through valve-switching. After that NO3- was collected in the sample loop and finally was injected into ion chromatography separation and detection. The operation was performed on Ion AS11-HC guard column (50 mm×4 mm) and analytical column (250 mm×4 mm),and KOH was as gradient elution. The content of NO2- was calculated indirectly by detection of NO3- in the sample after oxidation. Results: The calibration plots for NO2- were linear in the range from 0.01 to 1.00 mg·L-1 with good correlation (r=0.999 9). The relative standard deviation (RSD) was 2.3%(n=7), the limit of detection (LOD) was 8.57×10-4 mg·L-1. The recovery of nitrite in real samples were between 85.2% and 111.0%. The content of NO2- in 17 samples was 6.94-88.70 mg·L-1, and the other 5 samples were not detected. Conclusion: The method is accurate and sensitive, and could be applied to the determination of nitrite in salt.
TANG Jia-yi, ZHENG Yang-yang, HUANG Xue-ting, Nesterenko Pavel N, YE Ming-li, CHEN Mei-lan
. Determination of nitrite in salt by using H2O2 oxidation valve-switching ion chromatographic system[J]. Chinese Journal of Pharmaceutical Analysis, 2023
, 43(12)
: 2105
-2112
.
DOI: 10.16155/j.0254-1793.2023.12.16
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