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Vitamin A palmitate related substances identification by LC-MS techniques

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  • Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China

Received date: 2021-04-13

  Online published: 2024-06-21

Abstract

Objective: To identify the related substances of vitamin A palmitate(VAP) by LC-MS techniques. Methods: The separation was carried out on a Thermo BDS HYPERSIL C8(150 mm×4.6 mm, 2.4 μm)column using methanol, water and isopropanol as the mobile phases with gradient elution. The high resolution Q-TOF/MS operated in APCI and ESI mode were employed for the related substances accurate masses and elemental compositions determination of their parent ions and the product ions. The structures of the related substances contained in VAP and its stressed samples were then figured out through the elucidation of the UV spectra. Results: VAP and its related substances were adequately separated under the established HPLC conditions. A total of 22 major related substances in VAP and its stressed samples were detected and identified by the hyphenated techniques. Among them, 2 were listed in European Pharmacopoeia(EP) and the rest 20 were newly identified related pro-ducts. Conclusion: The established LC-MS method is effective for the separation and identification of the related substances of VAP, and the results were useful for its quality control.

Cite this article

CAI Yan-jiao, CHENG Ting-ting, HANG Tai-jun, SONG Min . Vitamin A palmitate related substances identification by LC-MS techniques[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(10) : 1782 -1795 . DOI: 10.16155/j.0254-1793.2021.10.16

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