Objective: To establish a rapid determination method for the total ash, acid insoluble ash and Danba residue in Aconiti Lateralis Radix Praeparata by near infrared spectroscopy (NIRS). Methods: 40 batches of samples were selected, including Baifu Pian, Heishun Pian and Shengfu Pian. After the samples were crushed, the near infrared spectra (NIRS) of the samples were collected by diffuse reflection. The scanning range was 1 950-1 550 cm-1, the scanning times were 32, the resolution was 16 cm-1, the temperature was (25±2) ℃, the relative humidity was(55±2)%, and each sample was scanned for 3 times. According to the general principles of Chinese Pharmacopoeia 2020, total ash and acid insoluble ash were determined, and the Danba residue was determined by chemical method. Kenston division method was used to divide the data set, of which 26 batches of samples were selected in the calibration set, and the remaining 14 batches were selected in the validation set. Partial least squares (PLS) models between total ash, acid insoluble ash, Danba residue and NIR spectral data were established. Results: The determination results of total ash, acid insoluble ash and Danba residue were 1.40%-15.4%, 0.008%-0.812%, and 0.044%-1.464%, respectively. The relative prediction deviation (RPD) of the total ash, acid insoluble ash and Danba residue models were 5.17, 3.47 and 3.41 respectively, and the correlation coefficient (rpre) of the validation set were 0.982 1, 0.978 0 and 0.975 0 respectively, indicating the predicted values of NIRS model and the measured values showed a good linear relation. Conclusion: The method of NIRS combined with PLS model can be used to quickly determine the total ash, acid insoluble ash and Danba residue in Aconiti Lateralis Radix Praeparata. The method is simple and has a certain guiding role in controlling the quality of Aconiti Lateralis Radix Praeparata.
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