Activity Analysis

L02 liver membrane bioaffinity material for rapid screening of lipid-lowering active constituents from Rheum palmatum L.*

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  • 1. Gansu University of Chinese Medicine, Lanzhou 730000, China;
    2. Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & MOE of PRC, Lanzhou 730000, China;
    3. Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine (TCM) of Colleges of Gansu Province, Lanzhou 730000, China;
    4. Wuxi the Second People’s Hospital, Wuxi 214000, China

Received date: 2021-08-29

  Online published: 2024-06-24

Abstract

Objective: To realize the high-throughput screening of active ingredients in natural products, a kind of bioaffinity material of L02 hepatic steatosis cell membrane was prepared by physical adsorption, and it was applied to the rapid screening of active components of Rheum palmatum L. for lowering blood lipid. Methods: Model of L02 liver steatosis cells was established by oleic acid. Bioaffinity material was prepared by physical adsorption of hydroxyl on silica gel and amino on cell membrane, and characterized by scan electron microscopy and infrared spectroscopy. The 30% ethanol extract of Rheum palmatum L. was adsorbed, and the adsorption results were analyzed by HPLC. Results: Scanning electron microscope confirmed that the surface of the material was covered with a membrane obviously. Representative peaks at 3 442.41, 1 549.35 and 1 106.23 cm-1 were assigned to the adsorption of vibration by -NH bond, indicating that the material was successfully prepared. Compared with bioaffinity materials of normal L02 cell membrane, bioaffinity material of L02 hepatic steatosis cell membrane specifically adsorbed 11 chemical components from 30% ethanol extract of Rheum palmatum L., respectively: aloe-emodin-8-O-β-D glucopyranoside, rhein-8-O-β-D glucopyranoside, emodin-8-O-β-D glucoside, physcion-8-O-β-D-monoglucoside, aloe-emodin, rhein, emodin, chrysophanol, physcion and two unknown components. Network pharmacology predicted the main targets of Rheum palmatum L. for lowering blood lipid were PPARα, HMGCR, AKT1, TNF, MTOR and APOE, etc. Molecular docking simulation showed that the binding ability of emodin, chrysophanol and physcion to PPARα protein was higher than that of HMGCR protein. Conclusion: The bioaffinity material of L02 hepatic steatosis cell membrane by physical adsorption can quickly screen out the hypolipidemic active components of Rheum palmatum L.. It can make up for the shortcoming of the combination of component separation and activity screening in traditional drug screening, and provides reference for the rapid screening of other active constituents of lipid-lowering of traditional Chinese medicine.

Cite this article

WU Xiao-yu, DUAN Wen-da, XIA Peng-fei, BIAN Hui-qin, WANG Yu-xia, LI Shao-hong, ZHAO Lei . L02 liver membrane bioaffinity material for rapid screening of lipid-lowering active constituents from Rheum palmatum L.*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(9) : 1511 -1521 . DOI: 10.16155/j.0254-1793.2022.09.04

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