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Quantitative analysis and network pharmacology research based on 6 components in Qingre Jiedu oral liquid*

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  • Department of Pharmacy, the Frist Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China

Received date: 2021-05-11

  Online published: 2024-06-24

Abstract

Objective: To establish an UPLC-MS/MS method for quantitative analysis of geniposide, chlorogenic acid, neochlorogenic acid, harpagide, gentiopicroside and baicalin in Qingre Jiedu oral liquid. And to investigate potential mechanism of pharmacology effect by approach of network pharmacology. Methods: Components were separated by a Phenomenex Kinetex C18 column (50 mm×2.1 mm, 2.6 μm), with mobile phase consisted of0.3% formic acid aqueous solution and 0.3% formic acid acetonitrile, using gradient elution. The flow rate was 0.4 mL·min-1. Column temperature was set as 40 ℃ and injection volume was 2 μL. Mass data acquisition was carried out in ESI- mode on an AB Sciex 4500 MD mass spectrometer (AB Sciex, Framinghan, MA, USA), using multiple reaction detection mode (MRM). The developed method was validated by aspects of specificity, precision, repeatability, stability, and accuracy. Network pharmacology analysis was conducted based on platform of Swiss Target Prediction, Drugbank, TTD, KEGG, Cytoscape software and so on. Results: The established method was well validated, with linearity range of geniposide, chlorogenic acid, neochlorogenic acid, harpagide, gentiopicroside and baicalin of 0.050-5.000 (r=0.998 1), 0.049-4.900 (r=0.998 4), 0.050-5.000 (r=0.998 3), 0.010-1.020 (r=0.997 3), 0.052-5.150(r=0.999 3) and 0.052-5.150 (r=0.997 9) μg·mL-1, respectively. The contents of baicalin in Qingrejiedu oral liquid of 3 different batches from 2 manufacturers all met the requirements of the Pharmacopoeia (>1.0 mg·mL-1). However,thecontents of geniposide, chlorogenic acid, neochlorogenic acid, and harpaside were quite different,presenting necessity for multicomponents quality control of Qingre Jiedu oral liquid. Network pharmacology study was carried out to explore potential pathways under effect of anti-inflammatory, anti-influenza, treatment of upper respiratory tract diseases, fever, dysphasia and thirst. Involved KEGG pathways were revealed as follows: IL-17 signaling pathway, cytotoxicity mediated by natural killer cells, NF-κB signaling pathway, anti-folic acid resistance pathway, inflammatory mediator regulation of TRP channel and others. Conclusion: The established UPLC-MS/MS method is fast and accurate for quality evaluation of Qingre Jiedu oral liquid. Network pharmacology analysis reveals its potential mechanism of action, verifying reliability of established quality method and benefitting clinical application of Qingre Jiedu oral liquid.

Cite this article

ZHU Xue-ya, LI Ze-yun, ZHANG Xiao, WEN Wei-hao, YUAN Yong-liang, TIAN Xin . Quantitative analysis and network pharmacology research based on 6 components in Qingre Jiedu oral liquid*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(9) : 1537 -1545 . DOI: 10.16155/j.0254-1793.2022.09.06

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