Objective: To develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method (HPLC-ESI-MS/MS) in order to qualitatively identify and quantitatively determine the ten components including three catechins, four flavonoids, two phenolic acids and one isocoumarin in Ardisiae Japonicae Herba from different habitats. Methods: Chromatographic separation was performed on a C18 column with linear gradient elution of methanol and 0.2‰ formic acid at a flow rate of 0.8 mL·min-1. The identification and quantification of those ten analytes were achieved on a hybrid quadrupole linear ion trap mass spectrometer. The detection was accomplished in the negative mode using multiple-reaction monitoring. The operating conditions were as follows: the ion spray voltage was -4 500 V; the turbo spray temperature was 650 ℃; nitrogen was used as the nebulizer gas (413 kPa) and heater gas (448 kPa); the curtain gas was kept at 172 kPa and interface heater was on. The results of different components were standardized by SPSS software, and analyzed using scatter diagram. Results: The linear relationships, linearity range, precision, stability, LOD and LOQ of the method were good for the 10 components. The average recoveries were found between 97.7%-103.6% and the precision in terms of RSDs were in the range of 0.43%-3.3%. The proposed method was successfully applied for the qualitative and quantitative analysis of ten chemical compositions in twenty-five batches of Ardisiae Japonicae Herba samples from different habitats which had great variation on the contents. The result of scatter diagram analysis demonstrated that the samples from authentic source (Hunan, Hubei and Guangdong) had closer relationship to the reference sample than samples from other sources. Conclusion: The method was simple, rapid, sensitive and specific. Simultaneous quantification of bioactive components by HPLC-ESI-MS/MS coupled with chemometric techniques would be a well-acceptable strategy to comprehensively control the quality of Ardisiae Japonicae Herba.
REN Yan-ping, ZHAO Ke-xin, WANG Sheng-xiang, WANG Qian, LI-Cen, SHI Rui, ZHANG Lan-tong
. Simultaneous determination of ten active components in Ardisiae Japonicae Herba by HPLC-ESI-MS/MS[J]. Chinese Journal of Pharmaceutical Analysis, 2022
, 42(9)
: 1561
-1569
.
DOI: 10.16155/j.0254-1793.2022.09.09
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