Safety Monitoring

Identification of mutual adulteration of Panax ginseng and Panax quinquefolius by multiplex ligation-dependent probe amplification combined with melting curve method*

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  • 1. Hubei Institute for Drug Control, NMPA Key Laboratory for Quality Control of Chinese Medicine, Wuhan 430075, China;
    2. Hubei University of Chinese Medicine, Wuhan 430060, China;
    3. School of Life Sciences, Hubei University, Wuhan 430062, China;
    4. College of Life Sciences, Wuhan University, Wuhan 430072, China

Revised date: 2022-04-17

  Online published: 2024-06-24

Abstract

Objective: To establish a rapid detection and identification method for mutual adulteration in prepared slices of Panax ginseng and Panax quinquefolius. Methods: Based on the principle of using multiplex ligation-dependent probe amplification (MLPA) to detect single nucleotide polymorphism (SNP) and point mutation in sequences, a specific probe was designed to find SNP sites in the 18S sequences of Panax ginseng and Panax quinquefolius for MLPA amplification. The melting temperature (Tm) was derived from the peak of the melting curve, and the differences in Tm values were used to identify Panax ginseng and Panax quinquefolius. Specificity, sensitivity and adulteration ratio experiments were performed to verify the feasibility of the method. Results: The specific melting curve Tm value of Panax ginseng probe was (79.3±0.2)℃, and that of Panax quinquefolius probe was (81.2±0.2)℃. When the ratio of Panax quinquefolius probe to Panax ginseng probe was 0.6∶1.0.Panax ginseng adulterated with 10% Panax quinquefolius, or Panax quinquefolius adulterated with 10% ginseng could be effectively detected. Conclusion: This study establishesa stable method for the identification of Panax ginseng adulterated with Panax quinquefolius, which is specific and sensitive.

Cite this article

WANG Wen-bin, MO Jing, HU Zhi-gang, DU Wei, WANG Bo . Identification of mutual adulteration of Panax ginseng and Panax quinquefolius by multiplex ligation-dependent probe amplification combined with melting curve method*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(9) : 1577 -1585 . DOI: 10.16155/j.0254-1793.2022.09.11

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