Chinese Journal of Pharmaceutical Analysis >

2023 , Vol. 43 >Issue 1: 77 - 84

DOI: https://doi.org/10.16155/j.0254-1793.2023.01.10

Ingredient Analysis

Simultaneous qualitative analysis of five alkaloids in Corydalis Rhizoma by the method of liner calibration with two reference substances*

Expand
  • 1. Zhejiang Institute for Food and Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Hangzhou 310052, China;
    2. National Institutes for Food and Drug Control, Beijing 100050, China

Received date: 2022-03-14

  Online published: 2024-06-24

Fold

Abstract

Objective: To establish a high performance liquid chromatographic method for the qualitative analysis of five alkaloids, namely, palmatine, berberine, dehydrocorydaline, tetrahydropalmatine and corydaline in Corydalis Rhizoma, and to investigate the feasibility of liner calibration with two reference substances(LCTRS) method for the qualitative analysis of the peaks. Methods: HPLC method was used. The mobile phase consisted of solvent A (acetonitrile) and solvent B (water containing 0.1% phosphoric acid, adjusted to pH 6.0 with triethylamine). The gradient elution(0-23 min, 20%A; 23-35 min, 20%A→30%A; 35-50 min, 30%A→80%A) was performed by HPLC at a flow rate of 1.0 mL·min-1. The column temperature was 35 ℃ and the detection wavelength was set at 280 nm. The injection volume was 5 μL. The actual retention time of five alkaloids in Corydalis Rhizoma was determined on 20 C18 columns of different brands or types. Two components, palmatine and corydaline, was selected as the two reference compounds. LCTRS method was used for accurately locating chromatographic peaks, and the method was verified on another three unknown columns. The retention time of the other four components were also predicted by the relative retention time method using the intermediate chromatographic peak of dehydrocorydaline as the single reference. The prediction accuracy and column compliance of these two methods were compared. Results: The five alkaloids were well separated in the 10 batches of samples. Among them, the peak prediction accuracy and column compliance of the LCTRS method were both 100%,which were significantly higher than those of the relative retention time method, and could better predict the retention time of the components in Corydalis Rhizoma on the unknown column. Conclusion: The established LCTRS method for the high-accuracy prediction of peak retention time can be used for the qualitative analysis of five alkaloids in Corydalis Rhizoma and is worthy of further promotion and application.

Key words： Corydalis Rhizoma; liner calibration with two reference substances(LCTRS); palmatine; berberine; dehydrocorydaline; tetrahydropalmatine; corydaline

Cite this article

ZHANG Lin-lin, DONG Ting, CHEN Bi-lian, ZHENG Cheng, SUN Lei, MA Lin-ke, MA Shuang-cheng . Simultaneous qualitative analysis of five alkaloids in Corydalis Rhizoma by the method of liner calibration with two reference substances*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(1) : 77 -84 . DOI: 10.16155/j.0254-1793.2023.01.10

References

[1] WANG Y, YU Y, SUN G, et al. Quality evaluation of powdered poppy capsule extractive by systematic quantified fingerprint method combined with quantitative analysis of multi-components by single marker method[J].J Pharm Biomed Anal, 2020, 185: 113247
[2] 姚静, 孙欣光, 董蓉, 等. HPLC-CAD一测多评法同时测定黄芪中6种成分含量[J].药学学报, 2021, 56(2):557
YAO J, SUN XG, DONG R, et al. Simultaneous quantitative analyses of six components in Astragalus membranaceus based on HPLC-CAD and quantitative analysis of multi-components with a single-marker[J].Acta Pharm Sin, 2021, 56(2):557
[3] 刘艳芬, 段芳, 张翘, 等. 基于一测多评(QAMS)法联合化学计量学的参贝止咳颗粒综合质量评价[J].中草药, 2022, 53(2):413
LIU YF, DUAN F, ZHANG Q, et al. Comprehensive quality evaluation of Shenbei Zhike granules based on HPLC-QAMS combined with chemometrics[J].Chin Tradit Herb Drugs, 2022, 53(2):413
[4] XIE J, WANG RY, YONG L, et al. Determination of nine nucleosides in Rhizoma Paridis by quantitative analysis of multi-components via a single marker method[J].J Sep Sci, 2021, 44(9):1866
[5] 唐登峰, 罗镭, 马临科, 等. 对照品替代法用于莪术油中3个倍半萜类成分的含量测定研究[J].中国现代应用药学, 2020, 37(4):447
TANG DF, LUO L, MA LK, et al. Studies on the determination of three sesquiterpenoids in the zedoary turmeric oil by substitution of reference substance method[J].Chin J Mod Appl Pharm, 2020, 37(4):447
[6] 孙磊, 叶六平, 于新兰, 等. HPLC相对保留时间法中参照物的优选[J].药物分析杂志, 2020, 40(3):420
SUN L, YE LP, YU XL, et al. Optimal selection of reference compound for HPLC relative retention time[J].Chin J Pharm Anal, 2020, 40(3):420
[7] 孙磊, 金红宇, 逄瑜, 等. 双标多测法Ⅰ―双标线性校正技术用于色谱峰的定性[J].药物分析杂志, 2013, 33(8):1424
SUN L, JIN HY, PANG Y, et al. Two reference substances for determination of multiple components(Ⅰ):linear calibration using two reference substances for identification of chromatographic peaks[J].Chin J Pharm Anal, 2013, 33(8):1424
[8] SUN L, JIN HY, TIAN RT, et al. A simple method for HPLC retention time prediction: linear calibration using two reference substances[J].Chin Med, 2017, 12: 16
[9] CHEN AZ, SUN L, HANG Y, et al. Simultaneous qualitative and quantitative analysis of 11 active compounds in Rhubarb using two reference substances by UHPLC[J].J Sep Sci, 2018, 41(19):3686
[10] 栾永福, 臧远芳, 刘洪超, 等. 基于双标线性校正法的银黄口服液多组分定性分析[J].药物分析杂志, 2021, 41(6):1091
LUAN YF, ZANG YF, LIU HC, et al. Qualitative determination of multiple components in Yinhuang oral liquid by linear calibration using two reference substances[J].Chin J Pharm Anal, 2021, 41(6):1091
[11] 周亚楠, 张元元, 李会军, 等. 双标线性校正技术用于天麻多成分色谱峰的定性分析[J].药学学报, 2021, 56(7):1797
ZHOU YN, ZHANG YY, LI HJ, et al. Identification of Gastrodiae Rhizoma chromatographic peaks identification by liner calibration with two reference substances assisted[J].Acta Pharm Sin, 2021, 56(7):1797
[12] 郭美玲, 翟宏宇, 赵琳琳, 等. 双标线性校正技术用于淫羊藿药材色谱峰的定性[J].中国药学杂志, 2021, 56(5):406
GUO ML, ZHAI HY, ZHAO LL, et al. Identification of Herba Epimedii chromatographic peaks by linear calibration with two reference substances[J].Chin Pharm J, 2021, 56(5):406
[13] 杨鑫宝, 刘扬子, 杨秀伟, 等. 磐安延胡索的化学成分研究[J].中草药, 2013, 44(16):2200
YANG XB, LIU YZ, YANG XW, et al. Study on chemical constituents from Corydalis Rhizoma in Pan'an[J].Chin Tradit Herb Drugs, 2013, 44(16):2200
[14] XU YJ, SUN JF, LI WW, et al. Analgesic effect of the main components of Corydalis yanhusuo(Yanhusuo in Chinese) is caused by inhibition of voltage gated sodium channels[J].J Ethnopharmacol, 2021, 280:114457
[15] 马宁宁, 李欣, 金华, 等. 延胡索不同提取物抗炎作用的谱效关系及机制研究[J].中草药, 2019, 50(10):2413
MA NN, LI X, JIN H, et al. Spectrum-effect relationship and mechanism of anti-inflammatory effects of different extracts of Corydalis yanhusuo[J].Chin Tradit Herb Drugs, 2019, 50(10):2413
[16] TAN CN, ZHANG Q, LI CH, et al. 2019. Potential target-related proteins in rabbit platelets treated with active monomers dehydrocorydaline and canadine from Rhizoma Corydalis[J].Phytomedicine, 2019, 54: 231
[17] LI QY, GUAN HY, WANG XM, et al. Fingerprint-efficacy study of the quaternary alkaloids in Corydalis yanhusuo[J].J Ethnopharmacol, 2017, 207: 108
[18] 中华人民共和国药典2020年版. 一部[S].2020: 145
ChP 2020. VolⅠ[S].2020: 145
[19] 陈俊, 许浚, 张静雅, 等. 基于一测多评法对延胡索中生物碱类成分的质量控制研究[J].中草药, 2016, 47(3):493
CHEN J, XU J, ZHANG JY, et al. Quality control for alkaloids of Corydalis Rhizoma based on quantitative analysis on multi-components with single marker[J].Chin Tradit Herb Drugs, 2016, 47(3):493
[20] 孙磊, 金红宇, 马双成, 等. 中药标准物质替代测定法技术指导原则[J].中国药学杂志, 2015, 50(4):284
SUN L, JIN HY, MA SC, et al. Guideline of substitute reference substance method for evaluation of traditional Chinese medicines[J].Chin Pharm J, 2015, 50(4):284
Options
Outlines

/

〈 〉

京ICP备17052540号-4
Copyright © Chinese Journal of Pharmaceutical Analysis, All Rights Reserved.
E-mail：ywfx@nifdc.org.cn
Powered by Beijing Magtech Co. Ltd.