Objective: To discuss the feasibility and potential application of nano-differential scanning fluorescence (nanoDSF)to detect the melting temperature(T_m) of adalimumab. Methods: Using nano-DSF technique to measure T_m of adalimumab mAb at 6 different concentrations including 0.25, 0.5, 1, 5, 10, 50 mg·mL^-1, parameters were set during the detection process: the temperature range was 25-95 ℃, the rate of temperature elevation was 1 ℃·min^-1, the intrinsic fluorescence signal was collected to measure T_m, and the detection was repeated for 5 times. The specificity of nano-DSF was inspected by comparing the T_m results of adalimumab mAb and its formulation buffer. The precision of nano-DSF was obtained by evaluating the results of 5 T_m test results of adalimumab mAb at 6 different concentrations. The detection accuracy of nano-DSF technology was assessed by comparasion with the T_m detection results of adalimumab mAb with the differential scanning calorimetry (DSC) under 3 concentrations including 0.25, 0.5, 1 mg·mL^-1. In the bases of method evaluation above, the technology was applied to evaluate the similarity between adalimumab mAb reference biotherapeutic product (RBP) and 6 similar biotherapeutic products (SBPs) T_m. Besides, the initial aggregation temperature (T_agg) of adalimumab mAb under 6 different concentrations was also detected by collecting the scattered light signals of antibody molecules during the heating process. Results: nano-DSF technique could specifically identify T_m of adalimumab but there was no characteristic curve of corresponding formulation buffer, indicating the specificity of this method. Besides the nano-DSF technique had good precision in detecting 6 samples with different concentrations of 0.25, 0.5, 1, 5, 10, 50 mg·mL^-1 and each sample was detected 5 times, and the RSDs of T_m values were within 1%. Compared with the results of differential scanning calorimetry (DSC), nano-DSF results were 2-3 ℃ lower generally, but the T_m measured by the two methods had good correlations(r≥0.99).The T_m maps of adalimumab mAb with different concentrations of 0.25, 0.5, 1, 5, 10, 50 mg·mL^-1 were basically the same by nano-DSF, and there was no obvious difference in the results of T_m1 and T_m3 while T_m2 had a tendency to decrease with increa-sing concentrations. In the similarity study of adalimumab mAb RBP and SBPs, the results of nano-DSF and DSC were also consistent.In addition, detecting the T_agg at the same time of T_m detection. The detected T_agg showed a trend of gradual decline with increasing concentrations, which played an important role in the interpretation of T_m detection results. Conclusion: nano-DSF technology has satisfactory specificity, precision, accuracy and wide concentration application range, thus ensuring the suitability in the thermal stability similarity study of RBP and SBPs, etc.