Objective: To develop a method for simultaneous determination of syringin, eleutheroside E, isofraxidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, gehydrotumulosic acid, gehydropachymic acid, pachymic acid and synonyms in Wujiashen oral liquid by RP-HPLC. Methods: The determination was performed on a SHIMADZU-GL Wondasil C18-WR column(250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile and 0.3% phosphoric acid in a gradient mode at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 203 nm.The column temperature was set at 35 ℃. Results: The linear ranges for syringin, eleutheroside E, isofraxidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, gehydrotumulosic acid, gehydropachymic acid, pachymic acid and synonyms were 10.74-80.55, 7.04-52.8, 2.932-21.99, 2.232-16.74, 5.84-43.8, 10.42-78.15, 2.544-19.08, 1.9-14.25, 3.272-24.54 and 0.642-4.815 μg·mL-1, respectively(r=0.999 3-0.999 8). Average recoveries were 99.7%, 99.8%, 99.9%, 100.2%, 99.8%, 100.0%, 99.9%, 100.0%, 99.9% and 99.7% (RSD＜1.0%,n=6), respectively. The average contents of the above 10 components in 3 samples were 1.357-1.423, 0.853-0.881, 0.353-0.364, 0.265-0.276, 0.731-0.748, 1.282-1.311, 0.322-0.331, 0.239-0.246, 0.391-0.411 and 0.079-0.081 mg·mL-1, respectively. Conclusion: The method is simple and suitable to control the quality of Wujiashen oral liquid.
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