Ingredient Analysis

Study on the chromatographic fingerprint and 10 components of Tibetan medicine Swertia franchetiana H. Smith by HPLC*

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  • 1. Xining Center for Food and Drug Inspection & Testing, Xining 810007, China;
    2. Qinghai Provincial Drug Evaluation and Verification Center, Xining 810007, China;
    3. S&P Pharmaceutical Co., Ltd., Xining 810016, China

Received date: 2022-10-12

  Online published: 2024-06-24

Abstract

Objective: To establish a method for simultaneous determination of fingerprint and multi-target ingredients and quality control and evaluation in Swertia franchetiana H.Smith by HPLC. Methods: The analysis was performed on Waters Sunfire ODS-C18 column with mobile phase consisting of methanol -0.05% phosphoric acid solution with gradient elution at 1.0 mL·min-1. The detection wavelength was 254 nm and the column temperature was 30 ℃. Samilarity evaluation combined with Chempattern chemical software was used to evaluate the fingerprint established by HPLC. The content of 10 index components were determined by HPLC. Results: Swertia franchetiana H.Smith fingerprint was established, similarity>0.904, 29 common peaks were confirmed. 10 chromatographic peaks of loganin, swertiamarin, gentiopicroside, sweroside, mangiferin, homoorientin, amarogentin, mercaptosid, 1,8-dihydroxy-3,7-dimethoxyxanketo, bellidifolin were identified,ten components showed good linear relationships(r≥0.999 3). The recoveries were 96.8%-99.3%, RSD<2.1%. The average content determination result were 0.16%, 3.63%, 0.23%, 0.38%, 0.24%, 0.26%, 0.07%, 1.13%, 0.08%, 0.25%. The results showed that the content of swertiamarin and mercaptosid was high. Conclusion: The HPLC fingerprint combined with the content determination method of multi index components is suitable for the quality control and evaluation of Swertia franchetiana H.Smith. This method is fast, simple, stable and reliable, and can provide a basis for establishing the quality standard of Tibetan medicine Swertia franchetiana H.Smith.

Cite this article

LI Wei-ye, LIU Xue-liang, LIU An-ping, SU Yuan, YANG Zeng-liang, HAN Da-bin, SHI Chun-lan, XU Wen-sheng, LIU Hai-qing . Study on the chromatographic fingerprint and 10 components of Tibetan medicine Swertia franchetiana H. Smith by HPLC*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(2) : 209 -218 . DOI: 10.16155/j.0254-1793.2023.02.04

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