Objective: To establish a method for the simultaneous determination of O-nornuciferine, quercetin-3-O-sycambinoside, hyperoside, isoquercitrin, quercetin-3-O-glucuronide, and nuciferine in water extract of lotus leaf. Methods: The column was Lamdo Stamsil C18 (250 mm×4.6 mm, 5 μm), the mobile phase was 0.1% phosphoric acid aqueous solution-acetonitrile in gradient mode at a flow rate of 0.6 mL·min-1. The detective wavelength was set at 270 nm. The column temperature was 25 ℃ and the injection volume was 5 μL. The relative correction factors of the other five components to O-nornuciferine were calculated and their contents were determined. Results: The linear relationships of O-nornuciferine, quercetin-3-O-sambulide, hyperoside, isoquercitrin, quercetin-3-O-glucuronide, and nuciferine were good (r≥0.999 5). The RSDs of precision, repeatability and stability tests were less than 3.0%. The average recovery was 94.6%-108.0% with RSDs within 0.95%-2.1%. The content of the six components in the water extract of lotus leaf was determined by QAMS was 0.23, 3.43, 1.69, 10.59, 41.23, 0.69 mg·g-1. Conclusion: The established method for the determination of 6 components in the water extract of lotus leaf is reliable and can be used for its quality control and pharmacodynamics research.
[1] 李敏, 赵振华, 玄静, 等. 荷叶化学成分及其药理作用研究进展[J].辽宁中医药大学学报, 2020, 22(1):135
LI M, ZHAO ZH, XUAN J, et al.Adances in studies on chemical constituents and pharmacological effects of lotus leaves[J].J Liaoning Univ Tradit Chin Med, 2020, 22(1):135
[2] 陈曦, 戚进.荷叶中黄酮和生物碱的研究进展[J].中国实验方剂学杂志, 2015, 21(18):211
CHEN X, QI J. Flavonoids and alkaloids in lotus leaves[J].Chin J Exp Tradit Med Form, 2015, 21(18):211
[3] 王婵, 杨颖博.荷叶的化学成分与药理活性研究进展[J].现代中药研究与实践, 2020, 34(4):74
WANG C, YANG YB. Research progress on chemical constituents and pharmacological activities of Nelumbinis Folium[J].Res Pract Chin Med, 2020, 34(4):74
[4] 蒋锡兰, 王伦, 李甫, 等. 荷叶的抗氧化活性成分[J].应用与环境生物学报, 2017, 23(1):89
JIANG XL, WANG L, LI F, et al. Antioxidant components of lotus leaves[J].J Appl Environ Biol, 2017, 23(1):89
[5] 王昊, 夏明辉, 庞旭, 等. 荷叶中苷类成分的分离鉴定[J].天津中医药大学学报, 2017, 36(5):368
WANG H, XIA MH, PANG X, et al. Isolation and identification of glycosides from the leaves of Nelumbo nucifera Gaertn. [J].J Tianjin Univ Tradit Chin Med, 2017, 36(5):368
[6] 黄秀琼, 卿志星, 曾建国.莲不同部位化学成分及药理作用研究进展[J].中草药, 2019, 50(24):6162
HUANG XQ, QING ZX, ZENG JG. Research advances on chemical constituents and pharmacological effects of various parts of Nelumbo nucifera[J].Chin Tradit Herb Drugs, 2019, 50(24):6162
[7] 刘佳, 朱建广, 孔令戈, 等. 荷叶水提取物的急性毒性及辅助降血脂功效研究[J].现代食品, 2021(9):85
LIU J, ZHU JG, KONG LG, et al. Study on acute toxicity and auxiliary hypolipidemic activity of lotus leaf aqueous extract[J].Mod Food, 2021(9):85
[8] 骆航, 周振华, 李华生, 等. HPLC-QAMS同时测定芪桑益肝丸中8种成分的含量[J].中国现代应用药学, 2021, 38(9):1049
LUO H, ZHOU ZH, LI HS, et al. Simultaneous determination of eight components in Qisang Yigan pills by HPLC-QAMS[J].Chin J Mod Appl Pharm, 2021, 38(9):1049
[9] 储成俭, 金雅.基于一测多评法对不同生长年限黄芪中黄酮类成分的分析研究[J].中药材, 2019, 42(8):1777
CHU CJ, JIN Y. Analysis of flavonoids in Astragalus Radix with different growth years based on QAMS[J].J Chin Med Mater, 2019, 42(8):1777
[10] 郑云枫, 李洋, 段伟萍, 等. 一测多评法测定黄芪中异黄酮及苷类成分[J].中草药, 2021, 52(10):3104
ZHENG YF, LI Y, DUAN WP, et al. Determination of isoflavonoids and glycosides in Astragali Radix by QAMS method[J].Chin Tradit Herb Drugs, 2021, 52(10):3104
[11] 田欢, 赵峰, 杨媛媛, 等. 一测多评法测定桑白皮中6个成分的含量[J].中药材, 2021, 44(4):924
TIAN H, ZHAO F, YANG YY, et al. Determination of six components in Cortex Mori on QAMS[J].J Chin Med Mater, 2021, 44(4):924
[12] 陈影, 吴婷, 姜超, 等. 一测多评法同时测定4种中药注射液中9种丹参酚酸[J].中成药, 2021, 43(3):590
CHEN Y, WU T, JIANG C, et al. Simultaneoous determination of nine salvianolic acids in four Chinese herbal injections by QAMS[J].Chin Tradit Pat Med, 2021, 43(3):590
[13] 杨莎, 宋忠兴, 唐志书, 等. 一测多评法同时测定阿娜尔妇洁液中7种成分[J].中草药, 2021, 52(12):3560
YANG S, SONG ZX, TANG ZS, et al. Simultaneous determination of seven constituents in A’naer vigills by QAMS[J].Chin Tradit Herb Drugs, 2021, 52(12):3560
[14] 嵇增云, 贾鑫, 赵海龙, 等. 一测多评法同时测定蒙药述达格-4中5个黄酮类成分的含量[J].药物分析杂志, 2021, 41(9):1605
JI ZY, JIA X, ZHAO HL, et al. Simultaneous determination of five flavonoids in Mongolian medicine Shudage-4 by QAMS[J].Chin J Pharm Anal, 2021, 41(9):1605
[15] 刘玉振, 许伟.一测多评法测定麦芪降糖丸中的7种成分[J].华西药学杂志, 2021, 36(4):473
LIU YZ, XU W. Determination of seven components in Maiqi Jiangtang pills by QAMS[J].West China J Pharm Sci, 2021, 36(4):473
[16] 张作华, 褚光松, 王海燕, 等. 一测多评法同时测定苍耳子中5个酚酸类成分的含量[J].药物分析杂志, 2021, 41(9):1612
ZHANG ZH, CHU GS, WANG HY, et al. Simultaneous determination of five phenolic acids in Xanthii Fructus by QAMS[J].Chin J Pharm Anal, 2021, 41(9):1612
