Metabolism Analysis

Study on intracellular uptake of curdione in platelets*

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  • 1. Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China;
    2. The Grade 3 Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, Hefei 230022, China;
    3. School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, China;
    4. School of Pharmacy, Anhui Medical University, Hefei 230032, China

Revised date: 2022-12-05

  Online published: 2024-06-24

Abstract

Objective: To provide experimental basis and further clarify the pathway of anti-platelet active components of Curcuma zedoaria by the study on the intracellular uptake of curdione by platelets. Methods: Establishment of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect the concentration of curdione in platelets and validation of the methodology. In vitro, the washed human platelets were co-cultured with different concentrations of curdione. LC-MS/MS was used to screen the optimal conditions for the uptake of curdione. In vivo blood samples were collected from mice orally administered with curdione and clinical patients dosed with Zedoary Turmeric oil injection. Platelets were extracted by stepwise centrifugation. Afterwards, LC-MS/MS was performed to detect intracellular curcumin concentrations in serum and platelets. Results: The results indicated that curdione in platelets had a good linear relationship in a range of 1-200 ng·mL-1 (r=0.997 5). The limit of detection was 0.5 ng·mL-1. The intra-and inter-batch precision were less than 5.0%,the accuracy were less than 8.8% and the average recoveries were 93.1%-110.2%. The results in vitro showed that curdione (1, 0.5, 0.25 mmol·L-1) could be detected in platelet lysates under the same platelet count condition. In vivo, the data showed that curdione was detected in platelet of mice and patients. These results indicated that curdione could be uptaken by platelets in vitro and in vivo. Conclusion: Platelets can intracellularly uptake curdione. It provides experimental basis for further study on the mechanism of antiplatelet effects of curdione.

Cite this article

TAN Ting-fei, CHENG Zi-wei, HAN Gong-wei, XIA Zi-tong, SONG Shuai, XIA Quan . Study on intracellular uptake of curdione in platelets*[J]. Chinese Journal of Pharmaceutical Analysis, 2023 , 43(2) : 253 -261 . DOI: 10.16155/j.0254-1793.2023.02.09

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